An unbiased template of the Drosophila brain and ventral nerve cord.

janelia7_blocks-janelia7_biblio_header | block

bioRxiv. 2018 Jul 25;. doi: 10.1101/376384

An unbiased template of the Drosophila brain and ventral nerve cord. Saalfeld LabRubin LabHarris LabFlyLightScientific Computing Software

Bogovic JA, Otsuna H, Heinrich L, Ito M, Jeter J, Meissner GW, Nern A, Colonell J, Malkesman O, Saalfeld S

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individuals neurons and neural subtypes. Brain templates are essential for comparative biological studies because they enable analyzing many individuals in a common reference space. Several central brain templates exist for Drosophila, but every one is either biased, uses sub-optimal tissue preparation, is imaged at low resolution, or does not account for artifacts. No publicly available Drosophila ventral nerve cord template currently exists. In this work, we created high-resolution templates of the Drosophila brain and ventral nerve cord using the best-available technologies for imaging, artifact correction, stitching, and template construction using groupwise registration. We evaluated our central brain template against the four most competitive, publicly available brain templates and demonstrate that ours enables more accurate registration with fewer local deformations in shorter time.

node:body | entity_field

janelia7_blocks-janelia7_biblio_authors | block

janelia7_blocks-janelia7_biblio_tools | block

Main Menu (Mobile)- Block

Main Menu - Block

An unbiased template of the Drosophila brain and ventral nerve cord.

Abstract

Janelia Authors