Imaging Cellular Proteins and Structures

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The Liver : Biology and Pathobiology. ;:965 - 978. doi: 10.1002/978111943681210.1002/9781119436812.ch72

Arias IM, Alter HJ, Boyer JL, Cohen DE, Shafritz DA, Thorgeirsson SS, Wolkoff AW, Weigel AV, Snapp EL

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Abstract

This chapter describes many of the technologies, which have the potential to provide new insights into fundamental aspects of liver biology. Imaging live liver tissue in an animal with multiphoton microscopy coupled with photoactivatable fluorescent proteins and/or additional fluorescent proteins could be used to follow the lineage and fates of individual transplanted stem cells or developing transgenic cells in liver. Proteins or other molecules are labeled with a dye that can be excited with light source. Cells and proteins are generally too small to detect with the naked eye, relatively transparent when imaged by light microscopy, and are highly dynamic. With the increased signal to noise, isotropic and volumetric imaging and high speeds lattice light sheet allows for 3D super‐resolution microscopy, as well. Photomultiplier tubes, while capable of detecting and counting single photons, are less useful for high‐speed imaging because they normally only detect a single pixel at a time.

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Imaging Cellular Proteins and Structures

Abstract

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