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Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.

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Nature Methods. 2020 Mar 02;17(3):287-290. doi: 10.1038/s41592-020-0762-7
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Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo. Janelia Experimental Technology
Wu J, Liang Y, Chen S, Hsu C, Chavarha M, Evans SW, Shi D, Lin MZ, Tsia KK, Ji N
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Abstract

Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.

PMID: 32123392 [PubMed - indexed for MEDLINE]

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