Rapid adaptive optical recovery of optimal resolution over large volumes.

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Nature Methods. 2014 Apr;11:625-8. doi: 10.1038/nmeth.2925
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Rapid adaptive optical recovery of optimal resolution over large volumes. Betzig Lab
Wang K, Milkie DE, Saxena A, Engerer P, Misgeld T, Bronner ME, Mumm J, Betzig E
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Abstract

Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.

PMID: 24727653 [PubMed - indexed for MEDLINE]

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Janelia Authors

Eric Betzig
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