Whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores.

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Nano Letters. 2018 Dec 10;19(1):500-5. doi: 10.1021/acs.nanolett.8b04385

Whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores. Lavis Lab

Spahn C, Grimm JB, Lavis LD, Lampe M, Heilemann M

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Abstract

We demonstrate STED microscopy of whole bacterial and eukaryotic cells using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging. We achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multi-color and live cell STED microscopy.

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Whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores.

Abstract

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