Computational methods for stitching, alignment, and artifact correction of serial section data.

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Methods in Cell Biology. ;152:261 - 276. doi: 10.1016/bs.mcb.2019.04.007

Computational methods for stitching, alignment, and artifact correction of serial section data. Saalfeld LabElectron Microscopy

Saalfeld S

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Abstract

Imaging large samples at the resolution offered by electron microscopy is typically achieved by sequentially recording overlapping tiles that are later combined to seamless mosaics. Mosaics of serial sections are aligned to reconstruct three-dimensional volumes. To achieve this, image distortions and artifacts as introduced during sample preparation or imaging need to be removed.

In this chapter, we will discuss typical sources of artifacts and distortion, and we will learn how to use the open source software TrakEM2 to correct them.

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Computational methods for stitching, alignment, and artifact correction of serial section data.

Abstract

Janelia Authors