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jRCaMP1 - Improved Red Protein Indicators for In Vivo Calcium Imaging

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Reagents
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jRCaMP1 - Improved Red Protein Indicators for In Vivo Calcium Imaging

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Calcium Indicators

Optical imaging of calcium dynamics using genetically encoded calcium indicators (GECIs) is a powerful tool for systems neuroscience. The current state-of-the-art GECIs emit green light (green GECIs) and are derived from Green Fluorescent Protein (GFP), but green fluorescence fails in a number of important experimental setups where tissue absorption is of concern, creating a need for red indicators.

Red GECIs may be used for:

  • Deep tissue imaging
  • Dual-color imaging
  • Parallel use with lightsensitive ion channel (ChR2)

Joint efforts at Janelia have generated high-sensitivity red GECIs based on RCaMP and R-GECO for in vivo imaging of neural activity. New mRuby-based RCaMP (jRCaMP1), and available below, and mApple-based R-GECO (jRGECO1 information page; jRGECO1 tool page) variants have improved sensitivity and kinetics. Sensitivity of jRGECO1a in vivo was similar to state-of-the-art GFP-based GCaMP6. Performance of both reagents has been characterized in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. These reagents are available and distributed through Addgene.org as plasmids, Penn Vector Core as viral vectors, and in flies from the Bloomington Stock Center.

Patent Status:

Issued U.S. Patents 9,644,007 and 10,053,492 

Opportunity

Available from Addgene for non-profit research. Available for sale from Vigene Biosciences. Commercial licenses also available.

For inquiries, please reference:

Janelia 2015-026

jRCaMP1 calcium indicators

Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

CMV-NES-jRCaMP1a

red, improved SNR, slower kinetics, bright

61562

Dana et al., 2016

CMV-NES-jRCaMP1b

red, improved SNR, improved dynamic range, bright

63136

Dana et al., 2016

 

Viruses available from Addgene

Name Addgene virus Reference

AAV1.Syn.NES.jRCaMP1a.WPRE.SV40

AAV1
AAV9
Dana et al., 2016

AAV1.CAG.Flex.NES.jRCaMP1a.WPRE.SV40

AAV1

Dana et al., 2016

AAV1.Syn.Flex.NES.jRCaMP1a.WPRE.SV40

AAV1
AAVrg

Dana et al., 2016

AAV1.Syn.NES.jRCaMP1b.WPRE.SV40

AAV1
AAV9

Dana et al., 2016

AAV1.CAG.Flex.NES.jRCaMP1b.WPRE.SV40

AAV1

Dana et al., 2016

AAV1.Syn.Flex.NES.jRCaMP1b.WPRE.SV40

AAV1

Dana et al., 2016

 

Flies available from Bloomington Stock Center:

Line name Insertion Site Bloomington Stock Number Reference Chr
PBac{w[+mC]=20XUAS-IVS-NES-jRCaMP1a-p10} VK00005 63792 Dana et al., 2016 3
PBac{w[+mC]=20XUAS-IVS-NES-jRCaMP1b-p10} VK00005 63793 Dana et al., 2016 3

 

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RCaMP1h and R-GECO1 structure and mutations
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Contact

Michael Perham
Director, Innovations and External Relations
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