Main Menu (Mobile)- Block

Main Menu - Block

jRGECO1 - Improved Red Protein Indicators for In Vivo Calcium Imaging

janelia7_blocks-janelia7_node_tool_type_list | block
Reagents
node_title | node_title

jRGECO1 - Improved Red Protein Indicators for In Vivo Calcium Imaging

node:body | entity_field

Calcium Indicators

Download our GECI Commercial Catalog!

Optical imaging of calcium dynamics using genetically encoded calcium indicators (GECIs) is a powerful tool for systems neuroscience. The current state-of-the-art GECIs emit green light (green GECIs) and are derived from Green Fluorescent Protein (GFP). Still, green fluorescence fails in several important experimental setups where tissue absorption is of concern, creating a need for red indicators.

Red GECIs may be used for:

  • Deep tissue imaging
  • Dual-color imaging
  • Parallel use with light-sensitive ion channel (ChR2)

Joint efforts at Janelia have generated high-sensitivity red GECIs based on RCaMP and R-GECO for in vivo imaging of neural activity. New mRuby-based RCaMP (jRCaMP GENIE Page; jRCaMP Tool Page), and mApple-based R-GECO (jRGECO1 GENIE page), available below, variants have improved sensitivity and kinetics. The sensitivity of jRGECO1a in vivo was similar to state-of-the-art GFP-based GCaMP6. The performance of both reagents has been characterized in cultured neurons and in mice, Drosophila, zebrafish, and C. elegans in vivo. These reagents are available and distributed through Addgene.org as plasmids, Penn Vector Core as viral vectors, and in flies from the Bloomington Stock Center.

Opportunity

Available for non-profit research from Addgene. Available for purchase from Vigene Biosciences. Commercial licenses are also available.

jRGECO1 calcium indicators

Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

CMV-NES-jRGECO1a

red, improved SNR

61563

Dana et al., 2016

 

Viruses available from University of Pennsylvania Viral Vector Core:

Name Penn Catalog Number Reference

AAV1.Syn.NES.jRGECO1a.WPRE.SV40

AV-1-PV3849

Dana et al., 2016

AAV1.CAG.Flex.NES.jRGECO1a.WPRE.SV40

not yet available

Dana et al., 2016

AAV1.Syn.Flex.NES.jRGECO1a.WPRE.SV40

AV-1-PV3848

Dana et al., 2016

AAV1.Syn.NES.jRGECO1b.WPRE.SV40

AV-1-PV3846

Dana et al., 2016

AAV1.CAG.Flex.NES.jRGECO1b.WPRE.SV40

not yet available

Dana et al., 2016

AAV1.Syn.Flex.NES.jRGECO1b.WPRE.SV40

not yet available

Dana et al., 2016

 

Mice available from The Jackson Laboratory:

Name Description Jax Stock Number Reference
Thy1-jRGECO1a-WPRE (founder line GP8.20) red fluorescent calcium indicator with high sensitivity detection of action potentials, variable neuronal expression pattern 030525 Dana et al., manuscript in preparation
Thy1-jRGECO1a-WPRE (founder line GP8.31) red fluorescent calcium indicator with high sensitivity detection of action potentials, variable neuronal expression pattern 030526 Dana et al., manuscript in preparation
Thy1-jRGECO1a-WPRE (founder line GP8.58) red fluorescent calcium indicator with high sensitivity detection of action potentials, variable neuronal expression pattern 030527 Dana et al., manuscript in preparation
Thy1-jRGECO1a-WPRE (founder line GP8.62) red fluorescent calcium indicator with high sensitivity detection of action potentials, variable neuronal expression pattern 030528 Dana et al., manuscript in preparation

 

 

Flies available from Bloomington Stock Center:

Line name Insertion Site Bloomington Stock Number Reference Chr
PBac{w[+mC]=20XUAS-IVS-NES-jRGECO1a-p10} VK00005 63794 Dana et al., 2016 3
PBac{w[+mC]=20XUAS-IVS-NES-jRGECO1b-p10} VK00005 63795 Dana et al., 2016 3

 

node:field_image | entity_field
node:field_label | entity_field
RCaMP1h and R-GECO1 structure and mutations
individual_tool_resources | views
janelia_helper-janelia7_publication_list | block
janelia7_blocks-janelia7_tool_collaborators | block
janelia7_blocks-janelia7_program_administration | block

Contact

Michael Perham
Head of Innovation Management
addthis-addthis_block | block