BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples.

janelia7_blocks-janelia7_biblio_header | block

Nature Methods. 2019 Sep;16(9):870-74. doi: 10.1038/s41592-019-0501-0

BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples. Cardona LabTillberg LabKeller Lab

Hörl D, Rusak F, Preusser F, Tillberg P, Randel N, Chhetri RK, Cardona A, Keller PJ, Harz H, Leonhardt H, Treier M, Preibisch S

PDF of Article

PDF of Supplement

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that consist of many unaligned three-dimensional image tiles, which must be reconstructed before analysis. We developed the BigStitcher software to address this challenge. BigStitcher enables interactive visualization, fast and precise alignment, spatially resolved quality estimation, real-time fusion and deconvolution of dual-illumination, multitile, multiview datasets. The software also compensates for optical effects, thereby improving accuracy and enabling subsequent biological analysis.