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FlyLight / Protocols
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The protocols used by FlyLight have been optimized to consistently produce high-quality anatomical data year after year by a team of highly skilled and dedicated technicians.
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Protocols
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The protocols used by FlyLight have been optimized to consistently produce high-quality anatomical data year after year by a team of highly skilled and dedicated technicians.
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Videos of our Techniques

These videos illustrate FlyLight methods of dissecting and mounting the central nervous system of larval and adult Drosophila.  Videos by FlyLight Team.

Adult Brain Dissection

The brain is removed from an anesthetized Drosophila melanogaster adult.

 

Adult CNS dissection

The central nervous system (brain and ventral nerve cord) is removed from an anesthetized Drosophila melanogaster adult.

 

Adult Mounting

Fixed adult Drosophila melanogaster central nervous systems are mounted on a poly-L-lysine coated coverslip (see Recipe - Poly-L-Lysine), using a mounting dish described on the Drosophila Mounting T-Dish page.

 

DPX Embedding

A coverslip with mounted central nervous systems is embedded in DPX on a glass slide (see DPX Mounting).

 

Larval Dissection

The central nervous system is removed from a Drosophila melanogaster third instar larva.

 

Larval Mounting

Fixed larval Drosophila melanogaster central nervous systems are mounted on a poly-L-lysine coated coverslip (see Recipe - Poly-L-Lysine), using a mounting dish described on the Drosophila Mounting T-Dish page.