PTR performs a variety of staining and imaging techniques to visualize the presence and distribution of molecules in tissues by light microscopy. These include protocols developed in Team Projects, FlyLight (see below) and MultiFISH. In addition to the dissection and staining of adult fly brains to identify specific neurons, we dissect various developmental stages of flies and other animals and tissues, such as zebrafish embryos or mouse salivary glands. The EASI-FISH technique was developed in the MultiFISH team project to detect the expression of various genes in the same neuron in mice. This technique involves expansion of tissues prior to fluorescence in situ hybridization, and it provides high resolution and sensitivity. We are applying this technique to mouse, zebrafish and fly tissues. Frequently, we adapt protocols to answer new biological questions. Our imaging techniques include confocal and lightsheet microscopy.
We also provide functional imaging (2-photon microscopy), leveraging experience accrued in the Fly Functional Connectome team project, as well as expert electrophysiology support for studies in flies and rodent brain slices.
For several years, PTR has been working closely with the FlyLight, whereby many of our team member were fully dedicated to this Team Project. FlyLight is nearing its completion. The same protocols are now offered by the “FlyLight Extension” (FLE) as part of Project Pipeline Support (PPS) services. PTR, as the keeper of technologies developed in Team Projects, will also continue to provide the same services and coordinate these with PPS/FLE as required. Similarly, we are liaising with other support teams, such as the Fly Facility or Anatomy and Histology, to facilitate Janelia science most effectively.
We are currently seeking new team members with interest in bioimage annotations, imaging, or animal behavior experiments.