The mouse embryo has long been central to the study of mammalian development. We developed a light-sheet microscope that adapts itself to the dramatic changes in size, shape and optical properties of the post-implantation mouse embryo and captures its development from gastrulation to early organogenesis at the cellular level. We furthermore developed a computational framework for reconstructing long-term cell tracks, cell divisions, dynamic fate maps and maps of tissue morphogenesis across the entire embryo. By jointly analyzing cellular dynamics in multiple embryos registered in space and time, we built a dynamic atlas of post-implantation mouse development that, together with our microscopy and computational methods, is provided as a resource.
The publication of our imaging and image analysis framework for studying mouse development is available from the literature section (McDole, Guignard, Amat, Berger, Malandain, Royer, Turaga, Branson and Keller 2018, Cell).
By clicking on the link below to download software, you agree to the terms of the Janelia Open-Source Software License linked here.