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Labs:
Project Teams:
Main Menu - Block
Labs:
Project Teams:
- Overview
- Anatomy and Histology
- Cryo-Electron Microscopy
- Electron Microscopy
- Flow Cytometry
- Gene Targeting and Transgenics
- High Performance Computing
- Immortalized Cell Line Culture
- Integrative Imaging
- Invertebrate Shared Resource
- Janelia Experimental Technology
- Mass Spectrometry
- Media Prep
- Molecular Genomics
- Stem Cell & Primary Culture
- Project Pipeline Support
- Project Technical Resources
- Quantitative Genomics
- Scientific Computing
- Viral Tools
- Vivarium
iATPSnFR2
Download our Reagents Catalog here.
iATPSnFR2 is a circularly permuted GFP-based ATP sensor engineered for rapid, high-sensitivity detection of ATP levels in living cells. With ratiometric readout options and subcellular targeting flexibility, it's ideal for neuroscience, cell biology, and metabolism research, and drug screening.
Atalanta plasmid technology
Overview:
Next-gen DNA plasmids (pJAT series) for CRISPR-Cas9 genome modification using homology-directed repair (HDR)—originally developed for Drosophila, adaptable to other organisms.
Key Benefits:
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High HDR Efficiency: ~25% efficiency in injected eggs—significantly higher than traditional methods.
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One-Step Assembly: Combines dsDNA synthesis and Gateway cloning for rapid, simplified plasmid construction.
jASAP voltage indicators
Progress in neuroscience demands the ability to record electrical activity optically in specifically tagged neurons in behaving animals. With that in mind, GENIE Project's accomplished researchers harnessed their skills in assay and reagent development to create a superior, persistently visible genetically encoded voltage indicator (GEVI).
Voltron and Positron
Download our Reagents Catalog here.
Voltron 2.0 and Positron 2.0 are state-of-the-art genetically encoded voltage indicators (GEVIs) for use in vivo or in vitro. Developed and optimized at Janelia for sensitivity, signal strength, and speed, they couple to Janelia Fluor® dyes via the HaloTag protein labeling system.
iGABASnFR2
Download our Reagents Catalog here.
iGluSnFR3
Download our Reagents Catalog here.
iGluSnFR3 is the current (2022) generation of Glutamate-Sensing Fluorescent Reporters developed at HHMI's Janelia Research Campus to image the most abundant neurotransmitter in the mammal brain with the greatest performance to date.
jGCaMP8 Calcium indicators
Download our Reagents Catalog here.
smartFISH
FISH Background
The JFX™ Dyes
Download our Janelia Fluor® Dye Catalog here.
Overview
The JFX dye platform enhances the performance of Janelia Fluor® dyes by introducing deuterium at key positions, boosting brightness and extending photostability without altering their spectral signatures or cell permeability.