JF-BAPTA indicators merge the fluorescence power of Janelia Fluor dyes with the Ca²⁺ sensitivity of BAPTA and the genetic targeting precision of HaloTag. This next-generation sensor platform delivers high-performance calcium imaging in cytoplasmic and subcellular environments.
A versatile platform enabling the rational design of super-resolution imaging dyes by amide-coupling rhodamines to coumarin auxiliaries. Depending on the rhodamine’s lactone–zwitterion equilibrium (KL–Z), the dyes become either photochromic (405 nm-activatable) or spontaneously blinking at physiological conditions.
Photoactivatable (PA) Janelia Fluor dyes combine small-molecule fluorophores' superior brightness, photostability, and cell permeability with precise light-controlled activation. By incorporating a diazoketone caging group onto Janelia Fluor scaffolds, these dyes allow for on-demand fluorescence activation, enabling high-resolution imaging at the single-molecule level.
Fluorination and structural tuning of rhodamine scaffolds have yielded a powerful new class of bright, red-shifted fluorescent dyes for live-cell and super-resolution microscopy.
This new generation of small-molecule fluorescent dyes, based on rational tuning of the lactone–zwitterion equilibrium (KL–Z), delivers high-performance tools for biological imaging. Dyes engineered with KL–Z values between 10⁻² and 10⁻³ offer exceptional cell permeability, fluorogenicity, and compatibility with super-resolution techniques.
The rsCaMPARI technology is a groundbreaking sensor technology that provides a reversible method for marking and tracking neuronal activity. Based on photoconvertible fluorescent proteins, traditional neuronal activity markers offer permanent and irreversible marking, which limits their utility in experiments requiring multiple snapshots of activity within the same sample. To address this limitation, rsCaMPARI employs a reversibly switchable fluorescent protein (rsFP) that can photoswitch between bright and dim states in response to different wavelengths of light.
The iATPSnFR2 is a next-generation ATP sensor that offers significant improvements over its predecessor, iATPSnFR1, for real-time tracking of ATP levels within subcellular compartments. By optimizing the linkers between its domains, iATPSnFR2 achieves a 5-6 fold enhancement in its dynamic range. This sensor utilizes a modified GFP inserted within the ATP-binding helices of a bacterial ATPase subunit, ensuring high specificity and sensitivity to ATP while effectively discriminating against other analytes.
The Atalanta technology is a significant genetic engineering advancement, simplifying cloning and improving the efficiency of CRISPR-Cas9 induced homology-directed repair (HDR) for genome modification of Drosophila and potentially other species. Current methods for cloning reagents used in CRISPR-Cas9 induced HDR involved multiple, inefficient cloning steps.
Progress in neuroscience demands the ability to record electrical activity optically in specifically tagged neurons in behaving animals. With that in mind, GENIE Project's accomplished researchers harnessed their skills in assay and reagent development to create a superior, persistently visible genetically encoded voltage indicator (GEVI).
Voltron 2.0 and Positron 2.0 are state-of-the-art genetically encoded voltage indicators (GEVIs) for use in vivo or in vitro. Developed and optimized at Janelia for sensitivity, signal strength, and speed, they couple to Janelia Fluor® dyes via the HaloTag protein labeling system.
