iATPSnFR2

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iATPSnFR2

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High-Performance Genetically Encoded Sensor for Intracellular ATP

iATPSnFR2 is a circularly permuted GFP-based ATP sensor engineered for rapid, high-sensitivity detection of ATP levels in living cells. With ratiometric readout options and subcellular targeting flexibility, it's ideal for neuroscience, cell biology, and metabolism research, and drug screening.

High Signal: ΔF/F ~12, optimized for live-cell imaging
Tunable Affinity: Three variants have ATP K_d values of 4 µM, 16 µM, and 500 µM
Optional Ratiometric Output: HaloTag, mIRFP, and mCherry fusion options for normalization
Fast Response: Fluorescence changes complete in <2 sec
Subcellular Precision: Reveals ATP consumption in cytosol, mitochondria, or single synaptic boutons

Performance Snapshot

Variant	Kd (ATP)	ΔF/F	Application Target
A95A.A119L	530 µM	~12	General cellular ATP observation
A95K	16 µM	~11	Low [ATP] systems
S29W.A95K	4 µM	~12	Low [ATP] compartments, in vitro studies