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Optical Imaging Across Biological Length Scales: From Nanometers to Millimeters

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Optical Imaging Across Biological Length Scales: From Nanometers to Millimeters

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June 13 - 25, 2021
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Hosted by Teng-Leong Chew, this 12-day bootcamp course is designed to demonstrate how biological queries and hypotheses steer experimental designs on various microscopy platforms and across length scales from molecules to small animals.

Participants will receive didactic instruction and hands-on experience with localization super-resolution microscopy, structured illumination microscopy, STED, pixel-reassignment methods for enhanced resolution, expansion microscopy, various light sheet microscopy methods, and label-free nonlinear microscopy. Participatory lab sessions will be conducted with biological samples from single cells to various model organisms, on commercial instruments as well as pre-commercial instruments developed at Janelia, including iPALM, MOSAIC, and SiMView. The course will put a strong emphasis on image processing and quantitative analyses using open source software, as well as proper methods for handling big data. 

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Applications are open to anyone interested in understanding the full spectrum of optical imaging capabilities, including graduate students, postdocs, independent scientists, faculty and core facility managers. To be considered, applicants must justify their need to learn imaging on multiple modalities, expand on prior imaging experience, and explain their motivation for taking the course and how it will help their work. The course is designed to provide advanced training in optical microscopy. Prior microscopy experience is strongly encouraged. 

Janelia will cover the cost of accommodation and meals. Travel expenses are not covered, but support is available for those in need. Participants are expected to stay for the duration.

Faculty Instructors

Teng-Leong Chew, Janelia Research Campus/HHMI
Elizabeth Hillman, Columbia University
Florian Jug, Max Planck Institute of Molecular Cell Biology and Genetics
Kate McDole, MRC Laboratory of Molecular Biology, UK
Stephan Preibisch, Janelia Research Campus/HHMI
Meredith Price, Bitplane
Stephan Saalfeld, Janelia Research Campus/HHMI
Paul Tillberg, Janelia Research Campus/HHMI
Members of the Advanced Imaging Center and Light Microscopy Facility, Janelia Research Campus/HHMI

Special Lectures

Ron Vale, Janelia Research Campus/HHMI, Keynote
Eric Betzig, University of California, Berkeley
Elizabeth Hillman, Columbia University
Luke Lavis, Janelia Research Campus/HHMI
Jennifer Lippincott-Schwartz, Janelia Research Campus/HHMI
Kate McDole, MRC Laboratory of Molecular Biology, UK


Lattice light sheet microscope (Betzig, Janelia)
Interferometric PALM (Hess, Janelia)
SCAPE (Hillman, Columbia Univ.)
Lightsheet Z.1 microscope (Zeiss)
Elyra-7 (Zeiss)
SiMView light sheet microscope (Keller, Janelia)
MOSAIC (Betzig, Janelia)
LSM 880 with AiryScan confocal microscope (Zeiss)
TCS SP8 STED (Leica)
Multimodal SIM (Gustafsson, Janelia)

Application Instructions

To be considered, applicants must apply online and provide:

  1. Research background and prior imaging experience
  2. Brief statement of interest/need