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For structural biologists, the first step in determining a protein's precise molecular structure is often the hardest: coaxing the protein to grow into the orderly, three-dimensional crystals that are the starting material for most structural studies. For particularly difficult cases, it can take years to generate usable crystals – and sometimes the protein never crystallizes despite intense effort. A new technique developed by Janelia researchers allows scientists to use electron beams to determine protein structure from tiny crystals.
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