Biotin-Janelia Fluor® Conjugates

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Biotin-Janelia Fluor® Conjugates

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Multifunctional Probes for Imaging and Affinity Capture

Overview

Biotin-Janelia Fluor® conjugates are cell-permeable, fluorescent ligands designed for dual-purpose applications: live-cell imaging and biochemical purification using the HaloTag platform.

Key Benefits

Live-Cell Compatible
- Rhodamine-derived scaffolds ensure efficient intracellular labeling and visualization.
Bright and Fluorogenic
- Enhanced brightness upon HaloTag binding enables high-contrast imaging.
Affinity Tag Built-In
- Streptavidin-compatible biotin moiety allows pulldown of HaloTag-tagged compartments.
Outperforms Commercial Ligands
- Demonstrated superior capture and permeability versus existing biotin-HaloTag compounds.

Spotlight Ligands

JF646-biotin: Bright far-red fluorogenic probe; excellent for mitochondrial capture
JF635-biotin: Red-shifted probe with high labeling efficiency
JF608-biotin: Balanced option for multi-organelle imaging
JF549-biotin: Limited to cell-surface due to poor permeability

Applications

Fluorescent labeling of intracellular HaloTag proteins
Streptavidin-mediated pulldown of labeled organelles (e.g., mitochondria)
Proteomic profiling, cell sorting, or target validation
High-content imaging followed by molecular capture

Reference: bioRxiv 2022.07.02.498544; doi: https://doi.org/10.1101/2022.07.02.498544

IP Coverage: US Patent Application 18/390,289

Janelia Tech ID: 2023-001

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Publications

Lavis Lab

Optimizing multifunctional fluorescent ligands for intracellular labeling

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Biotin-Janelia Fluor® Conjugates