Biotin-Janelia Fluor® Conjugates

janelia7_blocks-janelia7_node_tool_type_list | block

Reagents

node_title | node_title

Biotin-Janelia Fluor® Conjugates

node:body | entity_field

Multifunctional Biotin- and Drug-Janelia Fluor® Conjugates

Overview

Biotin-Janelia Fluor® conjugates are cell-permeable, fluorescent ligands designed for affinity purification using the HaloTag platform with easy confirmation using imaging. Drug-JF compounds can elicit the translocation of proteins to defined subcellular regions.

Key Benefits

Live-Cell Compatible
Rhodamine scaffolds ensure efficient intracellular labeling.
Bright and Fluorogenic
Enhanced brightness upon HaloTag binding enables high-contrast imaging.
Outperforms Commercial Biotin Ligands
Streptavidin-compatible biotin moiety allows pulldown of HaloTag-tagged compartments with superior capture compared to existing biotin-HaloTag ligands.
Allows Targeted Translocation of Proteins
Drug-JF conjugates facilitate targeted translocation of proteins to different subcellular regions.

Spotlight Ligands

JF646-biotin: Bright far-red fluorogenic probe; excellent for mitochondrial capture
JF635-biotin: Red-shifted probe with high labeling efficiency
JF608-biotin: Balanced option for multi-organelle imaging
JF549-biotin: Limited to cell-surface due to poor permeability

Applications

Biotin-JF646: Bright far-red fluorogenic probe; excellent for mitochondrial capture
Biotin-JF635: Far-red probe with high fluorogenicity and low background
Biotin-JF608: An alternative color that is useful in cells and in vivo
JQ1-JF635: Drives translocation of BRD4 to different subcellular regions

Reference: bioRxiv 2022; doi: https://doi.org/10.1101/2022.07.02.498544

IP Coverage: US Patent Application 18/390,289

Janelia Tech ID: 2023-001

node:field_image | entity_field