Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution.

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Nature Methods. 2020 Jan 01;17(1):68-71. doi: 10.1038/s41592-019-0641-2
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Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution. Hess Lab
Hayworth KJ, Peale D, Januszewski M, Knott GW, Lu Z, Xu CS, Hess HF
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Abstract

We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with <10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.

PMID: 31740820 [PubMed - indexed for MEDLINE]

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Janelia Authors

Kenneth Hayworth
C. Shan Xu
Harald Hess
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