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Equipment
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We have a full complement of state-of-the-art microscopes and multiple workstations dedicated to image processing. Our imaging capabilities support most experiments involving, but not limited to, fixed and live specimens, cultured cells, tissue slices, whole mouse brain, zebrafish and drosophila embryos, etc.

Zeiss LSM 880 with Airyscan (inverted confocal)

   
  • Inverted laser scanning confocal microscope equipped with two multi-Alkali PMTs and 32-channel spectral GaAsP PMTs (up to 13 fps)
  • Lasers: 405, 458, 488, 514, 561, 594, 633 nm, and X-Cite 120PC Series illuminator
  • Airyscan super-resolution capabilities:
    • Up to 1.7x better resolution (in all three dimensions)
    • Improved sensitivity with 4-8x higher SNR
    • More gentle excitation (less photobleaching and phototoxicity)
  • Spectral unmixing capabilities:
    • Acquire 5-8 dyes in parallel with spectral imaging
    • Linear unmixing post-acquisition segregates mixed fluorescence signals from dyes and cellular autofluorescence
  • Z-piezo control for fast acquisitions
  • Live cell imaging with temperature and CO2 control
  • Experiment designer for advanced acquisitions
  • Additional features include tiling, photomanipulation, definite focus, etc.

Zeiss LSM 800 (inverted confocal)

  • Inverted laser scanning confocal microscope equipped with three GaAsP PMTs​
  • Lasers: 405, 488, 560, 633 nm, and X-Cite 120Q Series illuminator
  • Experiment designer for advanced acquisitions
  • Additional features include advanced tiling module etc.

Zeiss LSM 880 NLO (upright confocal)

  • Upright confocal and two-photon (2PE) laser scanning microscope equipped with two multi-Alkali PMTs, 32-channel spectral GaAsP PMTs (up to 13 fps), and a BiG-2 non-descanned GaAsP PMT (2PE)
  • Lasers: 405, 458, 488, 514, 561, 594, 633, adjustable 690-1064 nm (Chameleon), and HXP 120 illuminator
  • Long working distance, water-dipping objectives
  • Spectral unmixing capabilities:
    • Acquire 5-8 dyes in parallel with spectral imaging
    • Linear unmixing post-acquisition segregates mixed fluorescence signals from dyes and cellular autofluorescence
  • Experiment designer for advanced acquisitions
  • Additional features include tiling, photomanipulation, etc.

Zeiss Lightsheet Z.1 (LSFM)

  • Dual-side illumination light sheet fluorescence microscope (LSFM) equipped with two liquid cooled pco.edge sCMOS cameras for high sensitivity and speed (up to 100 fps)
  • Lasers: 405, 445, 488, 514, 561, and 638 nm
  • 3D acquisition capabilities:
    • Thin optical sectioning (light sheet thickness ranges from 2-14 µm) with high SNR
    • High-speed imaging with very low phototoxicity and photobleaching
    • Multiple objectives (including long working distance, water-dipping objectives) for aqueous (n=1.33) or cleared (n=1.45) samples
    • Tailored for zebrafish and drosophila embryos, whole mouse brain, expanded samples, and transparent specimens (up to 5 mm)
  • Live cell imaging with temperature and CO2 control
  • Additional features include pivot scanning, tiling, multiview (360°) imaging, etc.

Custom-built Lattice Light-sheet (LLSM)

  • Two custom-built lattice light-sheet microscopes (LLSM) equipped with two Hamamatsu ORCA-Flash4.0 V3 sCMOS cameras for high sensitivity and speed (up to 100 fps)
  • Lasers: 405, 488, 561, and 642 nm
  • 3D acquisition capabilities:
    • Thin optical sectioning (light sheet thickness ranges from 400-800 nm) with high SNR and optimal resolution of 230 nm (lateral) and 370 nm (axial)
    • High-speed imaging with very low phototoxicity and photobleaching
    • Long working distance, water-dipping objective for aqueous samples (n=1.33)
    • Tailored for zebrafish and drosophila embryos, expanded samples, and transparent specimens (up to 4 mm)
  • Live cell imaging with temperature control
  • Additional features include advanced tiling etc.

Zeiss Axio Imager.Z1 with ApoTome.2 (upright widefield)

  • Upright widefield microscope with apotome attachment equipped with AxioCam MRc (color) and MRm (monochrome CCD) cameras for high speed (up to 48 fps)
  • Lasers: Colibri.2 multi-LED illumination system (365, 470, and 590 nm) and HXP 120 illuminator with multiple filter cube sets
  • Long working distance, water-dipping objectives
  • ApoTome.2 high-resolution capabilities:
    • Structured illumination to achieve thin optical sectioning and diffraction-limited images with high SNR
    • ​Tailored for cell cultures, tissue sections of variable thickness, and low-scattering samples
    • 3D reconstructions
  • Additional features include advanced tiling module, dark-field illumination, etc.

Nikon Eclipse Ti (inverted widefield)

  • Inverted widefield microscope equipped with an Andor Zyla sCMOS camera for high sensitivity and speed (up to 50 fps)
  • Lasers: Lumencor SPECTRA X LED light engine (V, B, C, G/Y, R, and NIR) with multiple filter sets
  • Live cell imaging with temperature and CO2 control
  • JOBS module for advanced acquisitions with High Content Analysis/Heat Map Analysis:
    • Create any custom acquisition
    • Automated screening of multiwell plates
    • Intelligent acquisition capable of modifying settings based on real-time image analysis and feedback
  • Additional features include advanced tiling, perfect focus system (PFS), etc.

TissueGnostics TissueFAXS 200 (widefield and confocal slide scanner)

  • Combination widefield and spinning disk confocal microscope operated as a whole slide digital scanner equipped with an Andor Zyla sCMOS camera for high sensitivity and speed
  • Lasers: Two Lumencor SPECTRA X LED light engines (V, B, C, G/Y, R, and NIR) with multiple filter sets
  • High-throughput capabilities including an autoloader cassette that holds up to 200 slides for unsupervised workflow
    • Faster acquisitions are possible by only imaging the tissue sections ("automated" detection)
  • Tailored for multi-color slide scanning, tissue cytometry, 5 µm optical sectioning for samples up to 50 µm-thick, and brightfield imaging of chromogenic stained samples
  • Additional features include digital stitching of whole slides, tools for cytoplasmic membrane or nuclear measurements, advanced image analysis software, etc.
    • Strataquest: Image visualization and analysis software for automated detection/cytometry and context-based quantification tasks across entire slides

3DHISTECH Pannoramic 250 Flash (widefield slide scanner)

  • Widefield microscope operated as a whole slide digital scanner equipped with a pco.edge sCMOS camera
  • Lasers: Lumencor SPECTRA LED light engine (V, B, C, G/Y, R, and NIR) with multiple filter sets
  • High-throughput capabilities including an autoloader cassette that holds up to 250 slides for unsupervised workflow
  • Tailored for multi-color slide scanning, tissue cytometry, and brightfield imaging of chromogenic stained samples
  • Additional features include digital stitching of whole slides, tools for cytoplasmic membrane or nuclear measurements, CaseCenter management system, etc.

Olympus MVX10 (upright macroscope)

  • Two upright macro zoom microscopes ("macroscopes") equipped with color CCD cameras
  • Lasers: BH2-RFL reflected light fluorescence attachment with multiple filter cube sets (DAPI, ECFP, GFP, Cy3, mRFP, mCherry, and Cy5)
  • Low and high (NA=0.5) magnification objectives for 50x zoom range without major changes in brightness
  • ​Tailored for dissection, long-term observation of whole organisms, and on-the-fly fluorescence imaging​

Olympus BX51 (upright compound)

  • Upright compound microscope equipped with a 12-bit Olympus DP71 color camera
  • 12V/100W halogen illumination source with multiple light filters and objectives including 100x/1.4 oil
  • ​Tailored for color histology/pathology samples and on-the-fly transmitted light viewing

Leica VT1000S (vibratome)

  • Two vibrating blade microtomes ("vibratomes") for high-quality sectioning
  • Section thickness ranges from 100-300 µm for live tissue and from 10-20 µm for fixed tissue
  • Tailored for neurophysiology, neuropathology, and experimental pathology applications