Bright, Photoactivatable Janelia Fluor Dyes for Super-Resolution Imaging

Overview

Photoactivatable (PA) Janelia Fluor dyes combine small-molecule fluorophores' superior brightness, photostability, and cell permeability with precise light-controlled activation. By incorporating a diazoketone caging group onto Janelia Fluor scaffolds, these dyes allow for on-demand fluorescence activation, enabling high-resolution imaging at the single-molecule level.

Key Advantages

• On-Demand Activation Activation by short-wavelength light (e.g., 405 nm) enables precise temporal and spatial control for single-particle tracking (sptPALM) and PALM imaging.
• Exceptional Brightness After activation, dyes achieve photon outputs comparable to or superior to those of leading fluorescent proteins like mEos3.2, delivering enhanced localization precision.
• Live-Cell and Fixed-Cell Compatibility Small, membrane-permeant ligands are compatible with HaloTag and SNAP-tag labeling strategies.
• Multicolor Capability Distinct spectral properties of PA-JF549 (green-orange) and PA-JF646 (far-red) allow for simultaneous two-color imaging in live or fixed cells.

Spotlight Dyes

• PA-JF549 Green-orange photoactivatable dye for high-precision localization and dynamic live-cell single-molecule tracking.
• PA-JF646 Far-red photoactivatable dye, ideal for deep-tissue imaging and multicolor super-resolution microscopy.

Applications

• Super-resolution imaging (PALM, sptPALM)
• Live-cell tracking of nuclear and cytoplasmic proteins
• Multicolor imaging experiments
• High-precision localization of intracellular structures (e.g., chromatin, mitochondria, cytoskeleton)

IP Coverage: US Patent 11,958,976, JP Application 2018-560894, EP Application EP17800333.1, CN 2017800283784

Opportunity: Available for non-exclusive commercial license