A split fluorescent reporter with rapid and reversible complementation.

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Nature communications. 2019 Jul;10:2822. doi: 10.1038/s41467-019-10855-0

A split fluorescent reporter with rapid and reversible complementation. Tebo Lab

Tebo AG, Gautier A

Note: Research in this publication was not performed at Janelia.

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Abstract

Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.

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A split fluorescent reporter with rapid and reversible complementation.

Note: Research in this publication was not performed at Janelia.

Abstract

Janelia Authors