Light sheet microscopy in cell biology.

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Methods in Molecular Biology. 2013;931:123-37. doi: 10.1007/978-1-62703-056-4_7

Light sheet microscopy in cell biology. Keller Lab

Tomer R, Khairy K, Keller PJ

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Abstract

Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth. This unique combination of capabilities makes light sheet-based microscopes highly suitable for live imaging applications. Here, we provide an overview of light sheet-based microscopy assays for in vitro and in vivo imaging of biological samples, including cell extracts, soft gels, and large multicellular organisms. We furthermore describe computational tools for basic image processing and data inspection.

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Light sheet microscopy in cell biology.

Abstract