Multiple new site-specific recombinases for use in manipulating animal genomes.

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Proceedings of the National Academy of Sciences of the United States of America. 2011 Aug 23;108(34):14198-203. doi: 10.1073/pnas.1111704108

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Multiple new site-specific recombinases for use in manipulating animal genomes. Svoboda LabRubin Lab

Nern A, Pfeiffer BD, Svoboda K, Rubin GM

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Abstract

Site-specific recombinases have been used for two decades to manipulate the structure of animal genomes in highly predictable ways and have become major research tools. However, the small number of recombinases demonstrated to have distinct specificities, low toxicity, and sufficient activity to drive reactions to completion in animals has been a limitation. In this report we show that four recombinases derived from yeast-KD, B2, B3, and R-are highly active and nontoxic in Drosophila and that KD, B2, B3, and the widely used FLP recombinase have distinct target specificities. We also show that the KD and B3 recombinases are active in mice.

PMID: 21831835 [PubMed - indexed for MEDLINE]

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Multiple new site-specific recombinases for use in manipulating animal genomes.

Abstract

PMID: 21831835 [PubMed - indexed for MEDLINE]

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