Main Menu (Mobile)- Block

Main Menu - Block

janelia7_blocks-janelia7_fake_breadcrumb | block
Koyama Lab / Publications
custom | custom


facetapi-Q2b17qCsTdECvJIqZJgYMaGsr8vANl1n | block
facetapi-PV5lg7xuz68EAY8eakJzrcmwtdGEnxR0 | block
facetapi-021SKYQnqXW6ODq5W5dPAFEDBaEJubhN | block
general_search_page-panel_pane_1 | views_panes

2 Publications

Showing 1-2 of 2 results
Your Criteria:
    08/01/84 | Analysis of P transposable element functions in Drosophila.
    Karess RE, Rubin GM
    Cell. 1984 Aug;38(1):135-46. doi: 10.1186/gb-2007-8-7-r145

    We have made a P-element derivative called Pc[ry], which carries the selectable marker gene rosy, but which acts like a nondefective, intact P element. It transposes autonomously into the germline chromosomes of an M-strain Drosophila embryo and it mobilizes in trans the defective P elements of the singed-weak allele. Frameshift mutations introduced into any of the four major open reading frames of the P sequence were each sufficient to eliminate the transposase activity, but none affected signals required in cis for transposition of the element. Complementation tests between pairs of mutant elements suggest that a single polypeptide comprises the transposase. We have examined transcripts of P elements both from natural P strains and from lines containing only nondefective Pc[ry] elements, and have identified two RNA species that appear to be specific for autonomous elements.

    View Publication Page
    03/01/84 | Precise identification of individual promoters for transcription of each strand of human mitochondrial DNA.
    Chang DD, Clayton DA
    Cell. 1984 Mar;36:635-43. doi: 10.1101/gad.1352105

    The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5’ end map positions to those reported for in vivo primary transcripts. An ordered series of deletion clones, whose template sequences were truncated at either the 5’ or 3’ end, was used to identify the precise mitochondrial DNA sequence required for initiation of transcription. The data provide a definitive assignment of the promoter for heavy-strand transcription occurring within -16 to +7 of the transcriptional start site 16 nucleotides upstream of the 5’ end of the gene for tRNAPhe and of the promoter for light-strand transcription occurring within -28 to +16 of the transcriptional start site at the 5’ end of "7S RNA." Within each control sequence is a candidate promoter whose consensus sequence is 5’-CANACC(G)CC(A)AAAGAPyA-3’ and in both cases transcriptional initiation occurs within six to eight nucleotides of the 3’ end of this sequence. The transcriptional start site is an integral part of each promoter and each promoter can function in the absence of the other.

    View Publication Page