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191 Publications
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01/01/20 |
Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution.
Nature Methods. 2020 Jan 01;17(1):68-71. doi: 10.1038/s41592-019-0641-2
We demonstrate gas cluster ion beam scanning electron microscopy (SEM), in which wide-area ion milling is performed on a series of thick tissue sections. This three-dimensional electron microscopy technique acquires datasets with <10 nm isotropic resolution of each section, and these can then be stitched together to span the sectioned volume. Incorporating gas cluster ion beam SEM into existing single-beam and multibeam SEM workflows should be straightforward, increasing reliability while improving z resolution by a factor of three or more.