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13 Publications

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    10/05/12 | Network resets in medial prefrontal cortex mark the onset of behavioral uncertainty.
    Karlsson MP, Tervo DG, Karpova AY
    Science. 2012 Oct 5;338:135-9. doi: 10.1126/science.1226518

    Regions within the prefrontal cortex are thought to process beliefs about the world, but little is known about the circuit dynamics underlying the formation and modification of these beliefs. Using a task that permits dissociation between the activity encoding an animal’s internal state and that encoding aspects of behavior, we found that transient increases in the volatility of activity in the rat medial prefrontal cortex accompany periods when an animal’s belief is modified after an environmental change. Activity across the majority of sampled neurons underwent marked, abrupt, and coordinated changes when prior belief was abandoned in favor of exploration of alternative strategies. These dynamics reflect network switches to a state of instability, which diminishes over the period of exploration as new stable representations are formed.

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    10/01/07 | Rapidly inducible, genetically targeted inactivation of neural and synaptic activity in vivo.
    Tervo D, Karpova AY
    Current Opinion in Neurobiology. 2007 Oct;17(5):581-6. doi: 10.1016/j.conb.2007.10.002

    Inducible and reversible perturbation of the activity of selected neurons in vivo is critical to understanding the dynamics of brain circuits. Several genetically encoded systems for rapid inducible neuronal silencing have been developed in the past few years offering an arsenal of tools for in vivo experiments. Some systems are based on ion-channels or pumps, others on G protein coupled receptors, and yet others on modified presynaptic proteins. Inducers range from light to small molecules to peptides. This diversity results in differences in the various parameters that may determine the applicability of each tool to a particular biological question. Although further development would be beneficial, the current silencing tool kit already provides the ability to make specific perturbations of circuit function in behaving animals.

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    Karpova LabSvoboda Lab
    12/08/05 | Rapid and reversible chemical inactivation of synaptic transmission in genetically targeted neurons.
    Karpova AY, Tervo DG, Gray NW, Svoboda K
    Neuron. 2005 Dec 8;48(5):727-35. doi: 10.1016/j.neuron.2005.11.015

    Inducible and reversible silencing of selected neurons in vivo is critical to understanding the structure and dynamics of brain circuits. We have developed Molecules for Inactivation of Synaptic Transmission (MISTs) that can be genetically targeted to allow the reversible inactivation of neurotransmitter release. MISTs consist of modified presynaptic proteins that interfere with the synaptic vesicle cycle when crosslinked by small molecule "dimerizers." MISTs based on the vesicle proteins VAMP2/Synaptobrevin and Synaptophysin induced rapid ( approximately 10 min) and reversible block of synaptic transmission in cultured neurons and brain slices. In transgenic mice expressing MISTs selectively in Purkinje neurons, administration of dimerizer reduced learning and performance of the rotarod behavior. MISTs allow for specific, inducible, and reversible lesions in neuronal circuits and may provide treatment of disorders associated with neuronal hyperactivity.

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