Fluorogenic Janelia Fluor Dyes

This new generation of small-molecule fluorescent dyes, based on rational tuning of the lactone–zwitterion equilibrium (K_L–Z), delivers high-performance tools for biological imaging. Dyes engineered with K_L–Z values between 10⁻² and 10⁻³ offer exceptional cell permeability, fluorogenicity, and compatibility with super-resolution techniques.

Key Benefits

• No-Wash Fluorogenic Imaging: Dyes are non-fluorescent until bound to targets, reducing background and simplifying workflows.
• Superior Cell Permeability: Lipophilic lactone states enhance membrane crossing, enabling live-cell intracellular labeling.
• Exceptional Brightness and Stability: Structural optimizations (e.g., azetidine substitution) yield high quantum yields and robust photostability.
• Advanced Microscopy Ready: Compatible with STED, SIM, lattice light-sheet, and SMLM imaging — including spontaneous blinking formats (e.g., HM-JF526).

Highlighted Molecules

• JF526: Bright green-emitting, highly fluorogenic dye for tagging proteins (HaloTag, SNAP-tag) and structures like microtubules, DNA, and lysosomes.
• HM-JF526: Hydroxymethyl derivative enabling spontaneous blinking for high-precision localization microscopy in standard imaging buffers.
• Other dye wavelengths are available at: JF646b, JF639b, JF639b, JF630b, JF629b, JF626b, and JF614b.

Applications

• Live-cell microscopy
• No-wash multicolor imaging
• Single-molecule super-resolution (SMLM)
• Drug discovery and target engagement assays
• Expand Your Imaging Capabilities.
• This technology platform offers tunable, modular fluorescent labels designed to meet the evolving demands of academic and industrial research.

IP Coverage: US Patent Application US17/027,286

Offer: Available for non-exclusive commercial license.