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Zeiss Lightsheet Z.1 Sample Holder

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Laboratory Tools
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Zeiss Lightsheet Z.1 Sample Holder

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Tool Description

Selective Plane Illumination Microscopy (SPIM) is a powerful tool for volumetric imaging of cleared specimens. The Zeiss Lightsheet microscope Z.1 offers a commercial solution for SPIM imaging. The Z.1 presents sample mounting challenges, though. For example, users must position samples vertically and stabilize them for hours-long imaging sessions. Therefore, we offer custom sample holder designs to mount cleared and expansion microscopy (ExM) samples on the Z.1 system.

Both 2021 and 2022 designs and fabrication instructions can be found in the Flintbox link to the right. 


Figure: Front view (A) and side view (B) of the sample holder with ExM sample attached.  The ExM sample was first mounted to a poly-L-lysine coated coverglass and then glued to the 3D-printed sample holder. Image credit: Mark Eddison.


The designs necessary to fabricate a sample holder are available for free at the Flintbox link to the right.

For other inquiries, please reference Tech ID: 2021-021

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Michael Perham
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