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Zeiss Lightsheet Z.1 Sample Holder
Selective Plane Illumination Microscopy (SPIM) is a powerful tool for volumetric imaging of cleared specimens. The Zeiss Lightsheet microscope Z.1 offers a commercial solution for SPIM imaging. The Z.1 presents sample mounting challenges, though. For example, users must position samples vertically and stabilize them for hours-long imaging sessions. Therefore, we offer custom sample holder designs to mount cleared and expansion microscopy (ExM) samples on the Z.1 system.
Figure: Front view (A) and side view (B) of the sample holder with ExM sample attached. The ExM sample was first mounted to a poly-L-lysine coated coverglass and then glued to the 3D-printed sample holder. Image credit: Mark Eddison.
The designs necessary to fabricate a sample holder are available for free at the Flintbox link to the right.