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Main Menu - Block
- Overview
- Anatomy and Histology
- Cell and Tissue Culture
- Cryo-Electron Microscopy
- Electron Microscopy
- Flow Cytometry
- Fly Facility
- Gene Targeting and Transgenics
- Janelia Experimental Technology
- Light Microscopy
- Media Prep
- Molecular Biology
- Project Pipeline Support
- Project Technical Resources
- Quantitative Genomics
- Scientific Computing Software
- Scientific Computing Systems
- Viral Tools
- Vivarium

Biography
BS from Cornell 1991-1995 (Biochemistry & Chemistry) PhD from Duke 1995-2001 (Computational Protein Engineering)Postdoc at Genentech 2001-2004 (Antibody Engineering by Phage Display and Computational PE)Senior Scientist at ImClone 2004-2006 (Antibody Engineering & Discovery)Senior Scientist at Janelia since 2006 (Biosensor design). Making intensity-based fluorescent biosensors from GFP and periplasmic binding proteins. When these proteins bind their small molecule ligand (maltose, glutamate, etc.), fluorescence increases by about 5-fold. We are making sensors for other neurotransmitters by using periplasmic binding proteins that already have the desired specificity and by designing new periplasmic binding proteins to accommodate ligands for which natural binding proteins do not exist. I use both computational protein design and library-based protein design (phage display and high throughout screening).