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Nature Communications. 2020 Jul 10;11(1)
A general approach to engineer positive-going eFRET voltage indicators Schreiter LabPodgorski LabGENIECell and Tissue CultureMolecular Genomics Viral Tools
Abdelfattah AS, Valenti R, Zheng J, Wong A, Podgorski K, Koyama M, Kim DS, Schreiter ER, GENIE Project
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Abstract
We engineered electrochromic fluorescence resonance energy transfer (eFRET) genetically encoded voltage indicators (GEVIs) with “positive-going” fluorescence response to membrane depolarization through rational manipulation of the native proton transport pathway in microbial rhodopsins. We transformed the state-of-the-art eFRET GEVI Voltron into Positron, with kinetics and sensitivity equivalent to Voltron but flipped fluorescence signal polarity. We further applied this general approach to GEVIs containing different voltage sensitive rhodopsin domains and various fluorescent dye and fluorescent protein reporters.
PMID: 32651384 [PubMed - indexed for MEDLINE]
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Previous bioRxiv PrePrint https://doi.org/10.1101/690925
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Janelia Authors
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