A complete electron microscopy volume of the brain of adult Drosophila melanogaster.

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Cell. 2018 Jul 12;174(3):730-43. doi: 10.1016/j.cell.2018.06.019

A complete electron microscopy volume of the brain of adult Drosophila melanogaster. Bock LabFetter LabSaalfeld LabBetzig LabHarris LabScientific Computing Software

Zheng Z, Lauritzen JS, Perlman E, Robinson CG, Nichols M, Milkie DE, Torrens O, Price J, Fisher CB, Sharifi N, Calle-Schuler SA, Kmecova L, Ali IJ, Karsh B, Trautman ET, Bogovic JA, Hanslovsky P, Jefferis GS, Kazhdan M, Khairy K

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Abstract

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly at synaptic resolution. To validate the dataset, we traced brain-spanning circuitry involving the mushroom body (MB), which has been extensively studied for its role in learning. All inputs to Kenyon cells (KCs), the intrinsic neurons of the MB, were mapped, revealing a previously unknown cell type, postsynaptic partners of KC dendrites, and unexpected clustering of olfactory projection neurons. These reconstructions show that this freely available EM volume supports mapping of brain-spanning circuits, which will significantly accelerate Drosophila neuroscience..

PMID: 30033368 [PubMed - indexed for MEDLINE]

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Additional authors: Saalfeld, Stephan; Fetter, Richard D; Bock, Davi D

First published in bioRxiv http://dx.doi.org/10.1101/140905 on June 13, 2017.

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A complete electron microscopy volume of the brain of adult Drosophila melanogaster.

Abstract

PMID: 30033368 [PubMed - indexed for MEDLINE]

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