Crystal structure of the nickel-responsive transcription factor NikR.

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Nature Structural Biology. 2003 Oct;10(10):794-9. doi: 10.1038/nsb985

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Crystal structure of the nickel-responsive transcription factor NikR. Schreiter Lab

Schreiter ER, Sintchak MD, Guo Y, Chivers PT, Sauer RT, Drennan CL

Note: Research in this publication was not performed at Janelia.

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Abstract

NikR is a metal-responsive transcription factor that controls nickel uptake in Escherichia coli by regulating expression of a nickel-specific ATP-binding cassette (ABC) transporter. We have determined the first two structures of NikR: the full-length apo repressor at a resolution of 2.3 A and the nickel-bound C-terminal regulatory domain at a resolution of 1.4 A. NikR is the only known metal-responsive member of the ribbon-helix-helix family of transcription factors, and its structure has a quaternary arrangement consisting of two dimeric DNA-binding domains separated by a tetrameric regulatory domain that binds nickel. The position of the C-terminal regulatory domain enforces a large spacing between the contacts that each NikR DNA-binding domain can make with the nik operator. The regulatory domain of NikR contains four nickel-binding sites at the tetramer interface, each exhibiting a novel square-planar coordination by three histidines and one cysteine side chain.

PMID: 12970756 [PubMed - indexed for MEDLINE]

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Crystal structure of the nickel-responsive transcription factor NikR.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 12970756 [PubMed - indexed for MEDLINE]

Janelia Authors