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1 Janelia Publications

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    Funke Lab
    02/04/20 | Reconstruction of motor control circuits in adult Drosophila using automated transmission electron microscopy
    Maniates-Selvin JT, Hildebrand DG, Graham BJ, Kuan AT, Thomas LA, Nguyen T, Buhmann J, Azevedo AW, Shanny BL, Funke J, Tuthill JC, Lee WA
    Cell. 2021 Feb 04;184(3):. doi: 10.1101/2020.01.10.902478

    Many animals use coordinated limb movements to interact with and navigate through the environment. To investigate circuit mechanisms underlying locomotor behavior, we used serial-section electron microscopy (EM) to map synaptic connectivity within a neuronal network that controls limb movements. We present a synapse-resolution EM dataset containing the ventral nerve cord (VNC) of an adult female Drosophila melanogaster. To generate this dataset, we developed GridTape, a technology that combines automated serial-section collection with automated high-throughput transmission EM. Using this dataset, we reconstructed 507 motor neurons, including all those that control the legs and wings. We show that a specific class of leg sensory neurons directly synapse onto the largest-caliber motor neuron axons on both sides of the body, representing a unique feedback pathway for fast limb control. We provide open access to the dataset and reconstructions registered to a standard atlas to permit matching of cells between EM and light microscopy data. We also provide GridTape instrumentation designs and software to make large-scale EM data acquisition more accessible and affordable to the scientific community.

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