Filter
Associated Lab
- Aso Lab (1) Apply Aso Lab filter
- Betzig Lab (1) Apply Betzig Lab filter
- Clapham Lab (1) Apply Clapham Lab filter
- Feliciano Lab (1) Apply Feliciano Lab filter
- Harris Lab (1) Apply Harris Lab filter
- Hess Lab (3) Apply Hess Lab filter
- Lavis Lab (1) Apply Lavis Lab filter
- Remove Lippincott-Schwartz Lab filter Lippincott-Schwartz Lab
- Liu (Zhe) Lab (5) Apply Liu (Zhe) Lab filter
- Rubin Lab (1) Apply Rubin Lab filter
- Saalfeld Lab (1) Apply Saalfeld Lab filter
- Singer Lab (1) Apply Singer Lab filter
Associated Support Team
Publication Date
- December 2019 (1) Apply December 2019 filter
- September 2019 (3) Apply September 2019 filter
- June 2019 (3) Apply June 2019 filter
- May 2019 (2) Apply May 2019 filter
- April 2019 (1) Apply April 2019 filter
- February 2019 (1) Apply February 2019 filter
- January 2019 (1) Apply January 2019 filter
- Remove 2019 filter 2019
12 Janelia Publications
Showing 11-12 of 12 resultsSeveral aquaporin (AQP) water channels are short-term regulated by the messenger cyclic adenosine monophosphate (cAMP), including AQP3. Bulk measurements show that cAMP can change diffusive properties of AQP3; however, it remains unknown how elevated cAMP affects AQP3 organization at the nanoscale. Here we analyzed AQP3 nano-organization following cAMP stimulation using photoactivated localization microscopy (PALM) of fixed cells combined with pair correlation analysis. Moreover, in live cells, we combined PALM acquisitions of single fluorophores with single-particle tracking (spt-PALM). These analyses revealed that AQP3 tends to cluster and that the diffusive mobility is confined to nanodomains with radii of ∼150 nm. This domain size increases by ∼30% upon elevation of cAMP, which, however, is not accompanied by a significant increase in the confined diffusion coefficient. This regulation of AQP3 organization at the nanoscale may be important for understanding the mechanisms of water AQP3-mediated water transport across plasma membranes.
Optical and electron microscopy have made tremendous inroads toward understanding the complexity of the brain. However, optical microscopy offers insufficient resolution to reveal subcellular details, and electron microscopy lacks the throughput and molecular contrast to visualize specific molecular constituents over millimeter-scale or larger dimensions. We combined expansion microscopy and lattice light-sheet microscopy to image the nanoscale spatial relationships between proteins across the thickness of the mouse cortex or the entire Drosophila brain. These included synaptic proteins at dendritic spines, myelination along axons, and presynaptic densities at dopaminergic neurons in every fly brain region. The technology should enable statistically rich, large-scale studies of neural development, sexual dimorphism, degree of stereotypy, and structural correlations to behavior or neural activity, all with molecular contrast.