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1 Janelia Publications

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    02/17/16 | Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy.
    Quirin S, Vladimirov N, Yang C, Peterka DS, Yuste R, Ahrens MB
    Optics Letters. 2016 Feb 17;41(5):855-8. doi: 10.1364/OL.41.000855

    Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning—removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160  μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain.

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