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1 Janelia Publications

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    Simpson Lab
    10/20/11 | Genetic manipulation of genes and cells in the nervous system of the fruit fly.
    Venken KJ, Simpson JH, Bellen HJ
    Neuron. 2011 Oct 20;72(2):202-30. doi: 10.1016/j.neuron.2011.09.021

    Research in the fruit fly Drosophila melanogaster has led to insights in neural development, axon guidance, ion channel function, synaptic transmission, learning and memory, diurnal rhythmicity, and neural disease that have had broad implications for neuroscience. Drosophila is currently the eukaryotic model organism that permits the most sophisticated in vivo manipulations to address the function of neurons and neuronally expressed genes. Here, we summarize many of the techniques that help assess the role of specific neurons by labeling, removing, or altering their activity. We also survey genetic manipulations to identify and characterize neural genes by mutation, overexpression, and protein labeling. Here, we attempt to acquaint the reader with available options and contexts to apply these methods.

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