Filter
Associated Lab
- Aso Lab (2) Apply Aso Lab filter
- Betzig Lab (1) Apply Betzig Lab filter
- Bock Lab (2) Apply Bock Lab filter
- Branson Lab (2) Apply Branson Lab filter
- Card Lab (1) Apply Card Lab filter
- Cardona Lab (4) Apply Cardona Lab filter
- Cui Lab (1) Apply Cui Lab filter
- Dickson Lab (1) Apply Dickson Lab filter
- Druckmann Lab (2) Apply Druckmann Lab filter
- Eddy/Rivas Lab (2) Apply Eddy/Rivas Lab filter
- Egnor Lab (1) Apply Egnor Lab filter
- Fetter Lab (3) Apply Fetter Lab filter
- Freeman Lab (1) Apply Freeman Lab filter
- Funke Lab (1) Apply Funke Lab filter
- Gonen Lab (1) Apply Gonen Lab filter
- Harris Lab (3) Apply Harris Lab filter
- Heberlein Lab (1) Apply Heberlein Lab filter
- Hess Lab (5) Apply Hess Lab filter
- Jayaraman Lab (3) Apply Jayaraman Lab filter
- Keller Lab (1) Apply Keller Lab filter
- Lavis Lab (1) Apply Lavis Lab filter
- Lee (Albert) Lab (1) Apply Lee (Albert) Lab filter
- Li Lab (1) Apply Li Lab filter
- Lippincott-Schwartz Lab (1) Apply Lippincott-Schwartz Lab filter
- Looger Lab (1) Apply Looger Lab filter
- Magee Lab (1) Apply Magee Lab filter
- Reiser Lab (1) Apply Reiser Lab filter
- Rubin Lab (7) Apply Rubin Lab filter
- Saalfeld Lab (6) Apply Saalfeld Lab filter
- Scheffer Lab (7) Apply Scheffer Lab filter
- Singer Lab (1) Apply Singer Lab filter
- Spruston Lab (2) Apply Spruston Lab filter
- Stern Lab (2) Apply Stern Lab filter
- Sternson Lab (1) Apply Sternson Lab filter
- Svoboda Lab (2) Apply Svoboda Lab filter
- Truman Lab (1) Apply Truman Lab filter
- Turner Lab (1) Apply Turner Lab filter
- Zlatic Lab (1) Apply Zlatic Lab filter
Associated Project Team
Associated Support Team
- Anatomy and Histology (1) Apply Anatomy and Histology filter
- Fly Facility (2) Apply Fly Facility filter
- Gene Targeting and Transgenics (1) Apply Gene Targeting and Transgenics filter
- Janelia Experimental Technology (3) Apply Janelia Experimental Technology filter
- Management Team (1) Apply Management Team filter
- Project Technical Resources (4) Apply Project Technical Resources filter
- Quantitative Genomics (1) Apply Quantitative Genomics filter
- Remove Scientific Computing Software filter Scientific Computing Software
- Scientific Computing Systems (1) Apply Scientific Computing Systems filter
Publication Date
53 Janelia Publications
Showing 1-10 of 53 resultsHow memories are used by the brain to guide future action is poorly understood. In olfactory associative learning in Drosophila, multiple compartments of the mushroom body act in parallel to assign valence to a stimulus. Here, we show that appetitive memories stored in different compartments induce different levels of upwind locomotion. Using a photoactivation screen of a new collection of split-GAL4 drivers and EM connectomics, we identified a cluster of neurons postsynaptic to the mushroom body output neurons (MBONs) that can trigger robust upwind steering. These UpWind Neurons (UpWiNs) integrate inhibitory and excitatory synaptic inputs from MBONs of appetitive and aversive memory compartments, respectively. After training, disinhibition from the appetitive-memory MBONs enhances the response of UpWiNs to reward-predicting odors. Blocking UpWiNs impaired appetitive memory and reduced upwind locomotion during retrieval. Photoactivation of UpWiNs also increased the chance of returning to a location where activation was initiated, suggesting an additional role in olfactory navigation. Thus, our results provide insight into how learned abstract valences are gradually transformed into concrete memory-driven actions through divergent and convergent networks, a neuronal architecture that is commonly found in the vertebrate and invertebrate brains.
Proteins localized at the cellular interface mediate cell-cell communication and thus control many aspects of physiology in multicellular organisms. Cell-surface proteomics allows biologists to comprehensively identify proteins on the cell surface and survey their dynamics in physiological and pathological conditions. PEELing provides an integrated package and user-centric web service for analyzing cell-surface proteomics data. With a streamlined and automated workflow, PEELing evaluates data quality using curated references, performs cutoff analysis to remove contaminants, connects to databases for functional annotation, and generates data visualizations. Together with chemical and transgenic tools, PEELing completes a pipeline making cell-surface proteomics analysis handy for every lab.
Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons in a brain and their connections. In order to exploit this knowledge base, researchers target individual neurons and study their function. Therefore, vast libraries of fly driver lines expressing fluorescent reporter genes in sets of neurons have been created and imaged using confocal light microscopy (LM). However, creating a fly line for driving gene expression within a single neuron found in the EM connectome remains a challenge, as it typically requires identifying a pair of fly lines where only the neuron of interest is expressed in both. This task and other emerging scientific workflows require finding similar neurons across large data sets imaged using different modalities. Here, we present NeuronBridge, a web application for easily and rapidly finding putative morphological matches between large datasets of neurons imaged using different modalities. We describe the functionality and construction of the NeuronBridge service, including its user-friendly GUI, data model, serverless cloud architecture, and massively parallel image search engine. NeuronBridge is openly accessible at http://neuronbridge.janelia.org/.
Cells contain hundreds of organelles and macromolecular assemblies. Obtaining a complete understanding of their intricate organization requires the nanometre-level, three-dimensional reconstruction of whole cells, which is only feasible with robust and scalable automatic methods. Here, to support the development of such methods, we annotated up to 35 different cellular organelle classes-ranging from endoplasmic reticulum to microtubules to ribosomes-in diverse sample volumes from multiple cell types imaged at a near-isotropic resolution of 4 nm per voxel with focused ion beam scanning electron microscopy (FIB-SEM). We trained deep learning architectures to segment these structures in 4 nm and 8 nm per voxel FIB-SEM volumes, validated their performance and showed that automatic reconstructions can be used to directly quantify previously inaccessible metrics including spatial interactions between cellular components. We also show that such reconstructions can be used to automatically register light and electron microscopy images for correlative studies. We have created an open data and open-source web repository, 'OpenOrganelle', to share the data, computer code and trained models, which will enable scientists everywhere to query and further improve automatic reconstruction of these datasets.
The neural circuits responsible for animal behavior remain largely unknown. We summarize new methods and present the circuitry of a large fraction of the brain of the fruit fly . Improved methods include new procedures to prepare, image, align, segment, find synapses in, and proofread such large data sets. We define cell types, refine computational compartments, and provide an exhaustive atlas of cell examples and types, many of them novel. We provide detailed circuits consisting of neurons and their chemical synapses for most of the central brain. We make the data public and simplify access, reducing the effort needed to answer circuit questions, and provide procedures linking the neurons defined by our analysis with genetic reagents. Biologically, we examine distributions of connection strengths, neural motifs on different scales, electrical consequences of compartmentalization, and evidence that maximizing packing density is an important criterion in the evolution of the fly's brain.
Animals avoid predators and find the best food and mates by learning from the consequences of their behavior. However, reinforcers are not always uniquely appetitive or aversive but can have complex properties. Most intoxicating substances fall within this category; provoking aversive sensory and physiological reactions while simultaneously inducing overwhelming appetitive properties. Here we describe the subtle behavioral features associated with continued seeking for alcohol despite aversive consequences. We developed an automated runway apparatus to measure how Drosophila respond to consecutive exposures of a volatilized substance. Behavior within this Behavioral Expression of Ethanol Reinforcement Runway (BEER Run) demonstrated a defined shift from aversive to appetitive responses to volatilized ethanol. Behavioral metrics attained by combining computer vision and machine learning methods, reveal that a subset of 9 classified behaviors and component behavioral features associate with this shift. We propose this combination of 9 be
Neurons perform computations by integrating inputs from thousands of synapses-mostly in the dendritic tree-to drive action potential firing in the axon. One fruitful approach to studying this process is to record from neurons using patch-clamp electrodes, fill the recorded neurons with a substance that allows subsequent staining, reconstruct the three-dimensional architectures of the dendrites, and use the resulting functional and structural data to develop computer models of dendritic integration. Accurately producing quantitative reconstructions of dendrites is typically a tedious process taking many hours of manual inspection and measurement. Here we present ShuTu, a new software package that facilitates accurate and efficient reconstruction of dendrites imaged using bright-field microscopy. The program operates in two steps: (1) automated identification of dendritic processes, and (2) manual correction of errors in the automated reconstruction. This approach allows neurons with complex dendritic morphologies to be reconstructed rapidly and efficiently, thus facilitating the use of computer models to study dendritic structure-function relationships and the computations performed by single neurons.
Studying the intertwined roles of sensation, experience, and directed action in navigation has been facilitated by the development of virtual reality (VR) environments for head-fixed animals, allowing for quantitative measurements of behavior in well-controlled conditions. VR has long featured in studies of Drosophila melanogaster, but these experiments have typically allowed the fly to change only its heading in a visual scene and not its position. Here we explore how flies move in two dimensions (2D) using a visual VR environment that more closely captures an animal's experience during free behavior. We show that flies' 2D interaction with landmarks cannot be automatically derived from their orienting behavior under simpler one-dimensional (1D) conditions. Using novel paradigms, we then demonstrate that flies in 2D VR adapt their behavior in response to optogenetically delivered appetitive and aversive stimuli. Much like free-walking flies after encounters with food, head-fixed flies exploring a 2D VR respond to optogenetic activation of sugar-sensing neurons by initiating a local search, which appears not to rely on visual landmarks. Visual landmarks can, however, help flies to avoid areas in VR where they experience an aversive, optogenetically generated heat stimulus. By coupling aversive virtual heat to the flies' presence near visual landmarks of specific shapes, we elicit selective learned avoidance of those landmarks. Thus, we demonstrate that head-fixed flies adaptively navigate in 2D virtual environments, but their reliance on visual landmarks is context dependent. These behavioral paradigms set the stage for interrogation of the fly brain circuitry underlying flexible navigation in complex multisensory environments.
It is unclear where in the nervous system evolutionary changes tend to occur. To localize the source of neural evolution that has generated divergent behaviors, we developed a new approach to label and functionally manipulate homologous neurons across Drosophila species. We examined homologous descending neurons that drive courtship song in two species that sing divergent song types and localized relevant evolutionary changes in circuit function downstream of the intrinsic physiology of these descending neurons. This evolutionary change causes different species to produce divergent motor patterns in similar social contexts. Artificial stimulation of these descending neurons drives multiple song types, suggesting that multifunctional properties of song circuits may facilitate rapid evolution of song types.
Animals are not simple input-output machines. Their responses to even very similar stimuli are variable. A key, long-standing question in neuroscience is to understand the neural correlates of such behavioral variability. To reveal these correlates, behavior and neural population activity must be related to one another on single trials. Such analysis is challenging due to the dynamical nature of brain function (e.g., in decision making), heterogeneity across neurons and limited sampling of the relevant neural population. By analyzing population recordings from mouse frontal cortex in perceptual decision-making tasks, we show that an analysis approach tailored to the coarse grain features of the dynamics is able to reveal previously unrecognized structure in the organization of population activity. This structure is similar on error and correct trials, suggesting dynamics that may be constrained by the underlying circuitry, is able to predict multiple aspects of behavioral variability and reveals long time-scale modulation of population activity.