Main Menu (Mobile)- Block
Main Menu - Block
janelia7_blocks-janelia7_fake_breadcrumb | block
janelia7_blocks-janelia7_support_team_header | block
What We Do
The Molecular Genomics team offers services and expert advice on routine and specialized molecular biology techniques.
node:body | entity_field
Equipment
-
Andrew plus pipetting robot
-
Bio-Rad ChemiDoc imaging station
-
Bio-Rad Gel Electrophoresis
-
Bio-Rad T100 Thermal cyclers
-
Bio-Rad Western Blot transfer
-
Eppendorf Gradient Thermal cycler
-
Helix agarose gel electrophoresis stations
-
QIAGEN QIAxcel Connect capillary electrophoresis
-
QIAGEN QIAcuity Digital PCR System
-
Single channel Nanodrops
-
ThermoFisher 2720 Thermal cyclers
-
Vizgen MERSCOPE
Services
Plasmid Prep Service
-
Endotoxin-free maxi plasmid preps
-
Whole plasmid sequencing with outside providers
Custom Cloning
-
Design, cloning, and validation of custom vectors using both in-house expertise and outside service providers
Genome Engineering
-
Design and prepare guideRNA, Cas enzyme, and homology-directed repair donor templates
-
Coordinate with non-rodent shared resources for delivery to embryo
-
Coordinate with shared resources for cell-line development
-
Mutation detection and identification
Spatial Transcriptomics
-
Vizgen MERSCOPE sample hybridization with probes and imaging
Resources
- qPCR and RT-qPCR gene expression analysis with commercial or custom assays
- Protein electrophoreses and Western blot analysis
- High volume protein production with outside providers
- Commonly used vectors for virus production, protein expression, and fluorescent protein labeling
janelia7_blocks-janelia7_block_right_hand_rail | block
