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Molecular Genomics

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More in this Support Team
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Support Teams / Molecular Genomics
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What We Do

Our Molecular Genomics team offers services and expert advice on routine and specialized molecular biology techniques. Our approach is driven by our passion for science, willingness to evolve our methods, and creative problem-solving abilities. We prioritize communication and collaboration to streamline the discovery process and achieve optimal results.

 

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Equipment

  • Andrew plus pipetting robot
  • Agilent 2100 Bioanalyzer
  • Bio-Rad ChemiDoc imaging station
  • Bio-Rad Gel Electrophoresis
  • Bio-Rad T100 Thermal cyclers
  • Bio-Rad Western Blot transfer
  • Eppendorf Gradient Thermal cycler
  • Helix agarose gel electrophoresis stations
  • Roche LC480 qPCR instrument
  • ThermoFisher 2720 Thermal cyclers
  • Single and multichannel Nanodrops

Services

Nucleic Acid Purification

  • Endotoxin-free DNA plasmid preps of all scales
  • Genomic DNA and RNA purification
  • In-vitro transcription of injection-quality RNA

Vector Construction

  • Design, cloning, and validation of custom vectors using both in-house expertise and outside service providers
  • Maintain a library of backbones commonly used for virus production, protein expression, and fluorescent protein labeling

Genome Engineering

  • Design and prepare guideRNA, Cas enzyme, and homology-directed repair donor templates
  • Coordinate with non-rodent shared resources for delivery to embryo
  • Mutation detection and identification

Gene Expression

  • qPCR and RT-qPCR with commercial or custom assays

Spatial Transcriptomics

  • Vizgen MERSCOPE sample hybridization with probes and imaging
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