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Molecular Biology

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Support Teams / Molecular Biology
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What We Do

Our Molecular Biology facility provides reliable services and expert assistance for routine and specialized molecular biology techniques. Critical to this effort are a passion for science, willingness to master new technologies, effective problem solving, and consistency in performance. We combine these elements with a customer-centric approach for scientific support to accelerate the discovery process.

 

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Equipment

  • 16 channel ABI 3130xl Genetic Analyzer for fast-turnaround DNA sequencing
  • MX 3005P real-time PCR instrument
  • Qiacube robot
  • Perkin Elmer Janus and EP3 Liquid handling robotics

 

Resources

Vector Construction

Cloning and vector construction services that encompass design, production and validation of custom vectors are available. The staff can work with you to design a strategy or carry out your cloning plan. We maintain a library of commonly used backbones and plasmid elements for applications that include virus production, protein expression and fluorescent protein tagging. The staff is experienced in a variety of cloning techniques as well as modifying inserts using site-directed mutagenesis. We can also acquire vectors from outside sources and manage outsourcing of vector construction projects to service providers.    

DNA Sequencing

The Molecular Biology Facility maintains and operates an ABI 3130xl DNA analyzer. This is a 16-capillary machine, capable of processing a maximum of ~384 sequencing reactions over 24 hours of continuous operation. The Facility also offers specialized sequencing services, such as transposon-mediated sequencing and direct sequencing from colonies using rolling circle amplification (RCA).

Nucleic Acid Isolation and Preparation

Plasmid DNA preps of all scales including endotoxin-free DNA preps are available. The facility can also provide post-purification services, such as restriction enzyme analysis, sequencing, and purification of inserts for cloning. We can also prepare in-vitro transcribed RNA for use in microinjection or in-situ hybridization applications.

Gene Expression and Quantitation services

The facility maintains a MX3005P real-time PCR instrument for quantitative expression analysis. The staff can also design and synthesis probes to validate expression patterns using in-situ hybridization.

Genotyping

Verification of transgenes through PCR based genotyping and sequencing is available.