Filter
Associated Lab
- Aguilera Castrejon Lab (16) Apply Aguilera Castrejon Lab filter
- Ahrens Lab (64) Apply Ahrens Lab filter
- Aso Lab (40) Apply Aso Lab filter
- Baker Lab (38) Apply Baker Lab filter
- Betzig Lab (113) Apply Betzig Lab filter
- Beyene Lab (13) Apply Beyene Lab filter
- Bock Lab (17) Apply Bock Lab filter
- Branson Lab (53) Apply Branson Lab filter
- Card Lab (42) Apply Card Lab filter
- Cardona Lab (64) Apply Cardona Lab filter
- Chklovskii Lab (13) Apply Chklovskii Lab filter
- Clapham Lab (15) Apply Clapham Lab filter
- Cui Lab (19) Apply Cui Lab filter
- Darshan Lab (12) Apply Darshan Lab filter
- Dennis Lab (1) Apply Dennis Lab filter
- Dickson Lab (46) Apply Dickson Lab filter
- Druckmann Lab (25) Apply Druckmann Lab filter
- Dudman Lab (50) Apply Dudman Lab filter
- Eddy/Rivas Lab (30) Apply Eddy/Rivas Lab filter
- Egnor Lab (11) Apply Egnor Lab filter
- Espinosa Medina Lab (19) Apply Espinosa Medina Lab filter
- Feliciano Lab (7) Apply Feliciano Lab filter
- Fetter Lab (41) Apply Fetter Lab filter
- Fitzgerald Lab (29) Apply Fitzgerald Lab filter
- Freeman Lab (15) Apply Freeman Lab filter
- Funke Lab (38) Apply Funke Lab filter
- Gonen Lab (91) Apply Gonen Lab filter
- Grigorieff Lab (62) Apply Grigorieff Lab filter
- Harris Lab (63) Apply Harris Lab filter
- Heberlein Lab (94) Apply Heberlein Lab filter
- Hermundstad Lab (27) Apply Hermundstad Lab filter
- Hess Lab (77) Apply Hess Lab filter
- Ilanges Lab (2) Apply Ilanges Lab filter
- Jayaraman Lab (46) Apply Jayaraman Lab filter
- Ji Lab (33) Apply Ji Lab filter
- Johnson Lab (6) Apply Johnson Lab filter
- Kainmueller Lab (19) Apply Kainmueller Lab filter
- Karpova Lab (14) Apply Karpova Lab filter
- Keleman Lab (13) Apply Keleman Lab filter
- Keller Lab (76) Apply Keller Lab filter
- Koay Lab (18) Apply Koay Lab filter
- Lavis Lab (149) Apply Lavis Lab filter
- Lee (Albert) Lab (34) Apply Lee (Albert) Lab filter
- Leonardo Lab (23) Apply Leonardo Lab filter
- Li Lab (28) Apply Li Lab filter
- Lippincott-Schwartz Lab (169) Apply Lippincott-Schwartz Lab filter
- Liu (Yin) Lab (6) Apply Liu (Yin) Lab filter
- Liu (Zhe) Lab (63) Apply Liu (Zhe) Lab filter
- Looger Lab (138) Apply Looger Lab filter
- Magee Lab (49) Apply Magee Lab filter
- Menon Lab (18) Apply Menon Lab filter
- Murphy Lab (13) Apply Murphy Lab filter
- O'Shea Lab (7) Apply O'Shea Lab filter
- Otopalik Lab (13) Apply Otopalik Lab filter
- Pachitariu Lab (48) Apply Pachitariu Lab filter
- Pastalkova Lab (18) Apply Pastalkova Lab filter
- Pavlopoulos Lab (19) Apply Pavlopoulos Lab filter
- Pedram Lab (15) Apply Pedram Lab filter
- Podgorski Lab (16) Apply Podgorski Lab filter
- Reiser Lab (51) Apply Reiser Lab filter
- Riddiford Lab (44) Apply Riddiford Lab filter
- Romani Lab (43) Apply Romani Lab filter
- Rubin Lab (143) Apply Rubin Lab filter
- Saalfeld Lab (63) Apply Saalfeld Lab filter
- Satou Lab (16) Apply Satou Lab filter
- Scheffer Lab (36) Apply Scheffer Lab filter
- Schreiter Lab (67) Apply Schreiter Lab filter
- Sgro Lab (21) Apply Sgro Lab filter
- Shroff Lab (31) Apply Shroff Lab filter
- Simpson Lab (23) Apply Simpson Lab filter
- Singer Lab (80) Apply Singer Lab filter
- Spruston Lab (93) Apply Spruston Lab filter
- Stern Lab (156) Apply Stern Lab filter
- Sternson Lab (54) Apply Sternson Lab filter
- Stringer Lab (35) Apply Stringer Lab filter
- Svoboda Lab (135) Apply Svoboda Lab filter
- Tebo Lab (33) Apply Tebo Lab filter
- Tervo Lab (9) Apply Tervo Lab filter
- Tillberg Lab (21) Apply Tillberg Lab filter
- Tjian Lab (64) Apply Tjian Lab filter
- Truman Lab (88) Apply Truman Lab filter
- Turaga Lab (51) Apply Turaga Lab filter
- Turner Lab (38) Apply Turner Lab filter
- Vale Lab (7) Apply Vale Lab filter
- Voigts Lab (3) Apply Voigts Lab filter
- Wang (Meng) Lab (21) Apply Wang (Meng) Lab filter
- Wang (Shaohe) Lab (25) Apply Wang (Shaohe) Lab filter
- Wu Lab (9) Apply Wu Lab filter
- Zlatic Lab (28) Apply Zlatic Lab filter
- Zuker Lab (25) Apply Zuker Lab filter
Associated Project Team
- CellMap (12) Apply CellMap filter
- COSEM (3) Apply COSEM filter
- FIB-SEM Technology (3) Apply FIB-SEM Technology filter
- Fly Descending Interneuron (11) Apply Fly Descending Interneuron filter
- Fly Functional Connectome (14) Apply Fly Functional Connectome filter
- Fly Olympiad (5) Apply Fly Olympiad filter
- FlyEM (53) Apply FlyEM filter
- FlyLight (49) Apply FlyLight filter
- GENIE (46) Apply GENIE filter
- Integrative Imaging (4) Apply Integrative Imaging filter
- Larval Olympiad (2) Apply Larval Olympiad filter
- MouseLight (18) Apply MouseLight filter
- NeuroSeq (1) Apply NeuroSeq filter
- ThalamoSeq (1) Apply ThalamoSeq filter
- Tool Translation Team (T3) (26) Apply Tool Translation Team (T3) filter
- Transcription Imaging (49) Apply Transcription Imaging filter
Publication Date
- 2025 (126) Apply 2025 filter
- 2024 (216) Apply 2024 filter
- 2023 (160) Apply 2023 filter
- 2022 (193) Apply 2022 filter
- 2021 (194) Apply 2021 filter
- 2020 (196) Apply 2020 filter
- 2019 (202) Apply 2019 filter
- 2018 (232) Apply 2018 filter
- 2017 (217) Apply 2017 filter
- 2016 (209) Apply 2016 filter
- 2015 (252) Apply 2015 filter
- 2014 (236) Apply 2014 filter
- 2013 (194) Apply 2013 filter
- 2012 (190) Apply 2012 filter
- 2011 (190) Apply 2011 filter
- 2010 (161) Apply 2010 filter
- 2009 (158) Apply 2009 filter
- 2008 (140) Apply 2008 filter
- 2007 (106) Apply 2007 filter
- 2006 (92) Apply 2006 filter
- 2005 (67) Apply 2005 filter
- 2004 (57) Apply 2004 filter
- 2003 (58) Apply 2003 filter
- 2002 (39) Apply 2002 filter
- 2001 (28) Apply 2001 filter
- 2000 (29) Apply 2000 filter
- 1999 (14) Apply 1999 filter
- 1998 (18) Apply 1998 filter
- 1997 (16) Apply 1997 filter
- 1996 (10) Apply 1996 filter
- 1995 (18) Apply 1995 filter
- 1994 (12) Apply 1994 filter
- 1993 (10) Apply 1993 filter
- 1992 (6) Apply 1992 filter
- 1991 (11) Apply 1991 filter
- 1990 (11) Apply 1990 filter
- 1989 (6) Apply 1989 filter
- 1988 (1) Apply 1988 filter
- 1987 (7) Apply 1987 filter
- 1986 (4) Apply 1986 filter
- 1985 (5) Apply 1985 filter
- 1984 (2) Apply 1984 filter
- 1983 (2) Apply 1983 filter
- 1982 (3) Apply 1982 filter
- 1981 (3) Apply 1981 filter
- 1980 (1) Apply 1980 filter
- 1979 (1) Apply 1979 filter
- 1976 (2) Apply 1976 filter
- 1973 (1) Apply 1973 filter
- 1970 (1) Apply 1970 filter
- 1967 (1) Apply 1967 filter
Type of Publication
4108 Publications
Showing 2611-2620 of 4108 resultsIn insects, the neuropeptide eclosion hormone (EH) acts on the CNS to evoke the stereotyped behaviors that cause ecdysis, the shedding of the cuticle at the end of each molt. Concomitantly, EH induces an increase in cyclic GMP (cGMP). Using antibodies against this second messenger, we show that this increase is confined to a network of 50 peptidergic neurons distributed throughout the CNS. Increases appeared 30 min after EH treatment, spread rapidly throughout these neurons, and were extremely long lived. We show that this response is synaptically driven, and does not involve the soluble, nitric oxide (NO)-activated, guanylate cyclase. Stereotyped variations in the duration of the cGMP response among neurons suggest a role in coordinating responses having different latencies and durations.
Measuring the dynamics of neural processing across time scales requires following the spiking of thousands of individual neurons over milliseconds and months. To address this need, we introduce the Neuropixels 2.0 probe together with newly designed analysis algorithms. The probe has more than 5000 sites and is miniaturized to facilitate chronic implants in small mammals and recording during unrestrained behavior. High-quality recordings over long time scales were reliably obtained in mice and rats in six laboratories. Improved site density and arrangement combined with newly created data processing methods enable automatic post hoc correction for brain movements, allowing recording from the same neurons for more than 2 months. These probes and algorithms enable stable recordings from thousands of sites during free behavior, even in small animals such as mice.
Although CMOS fabrication has enabled a quick evolution in the design of high-density neural probes and neural-recording chips, the scaling and miniaturization of the complete data-acquisition systems has happened at a slower pace. This is mainly due to the complexity and the many requirements that change depending on the specific experimental settings. In essence, the fundamental challenge of a neural-recording system is getting the signals describing the largest possible set of neurons out of the brain and down to data storage for analysis. This requires a complete system optimization that considers the physical, electrical, thermal and signal-processing requirements, while accounting for available technology, manufacturing constraints and budget. Here we present a scalable and open-standards-based open-source data-acquisition system capable of recording from over 10,000 channels of raw neural data simultaneously. The components and their interfaces have been optimized to ensure robustness and minimum invasiveness in small-rodent electrophysiology.
High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations, and is especially powerful when combined with optogenetics for manipulation of specific cell types with high temporal resolution. We integrated these approaches into prototype Neuropixels Opto probes, which combine electronic and photonic circuits. These devices pack 960 electrical recording sites and two sets of 14 light emitters onto a 1 cm shank, allowing spatially addressable optogenetic stimulation with blue and red light. In mouse cortex, Neuropixels Opto probes delivered high-quality recordings together with spatially addressable optogenetics, differentially activating or silencing neurons at distinct cortical depths. In mouse striatum and other deep structures, Neuropixels Opto probes delivered efficient optotagging, facilitating the identification of two cell types in parallel. Neuropixels Opto probes represent an unprecedented tool for recording, identifying, and manipulating neuronal populations.
Neuroscientists are now able to acquire data at staggering rates across spatiotemporal scales. However, our ability to capitalize on existing datasets, tools, and intellectual capacities is hampered by technical challenges. The key barriers to accelerating scientific discovery correspond to the FAIR data principles: findability, global access to data, software interoperability, and reproducibility/re-usability. We conducted a hackathon dedicated to making strides in those steps. This manuscript is a technical report summarizing these achievements, and we hope serves as an example of the effectiveness of focused, deliberate hackathons towards the advancement of our quickly-evolving field.
The accumulation of amyloid-beta (Abeta) into plaques is a hallmark feature of Alzheimer’s disease (AD). While amyloid precursor protein (APP)-related proteins are found in most organisms, only Abeta fragments from human APP have been shown to induce amyloid deposits and progressive neurodegeneration. Therefore, it was suggested that neurotoxic effects are a specific property of human Abeta. Here we show that Abeta fragments derived from the Drosophila orthologue APPL aggregate into intracellular fibrils, amyloid deposits, and cause age-dependent behavioral deficits and neurodegeneration. We also show that APPL can be cleaved by a novel fly beta-secretase-like enzyme. This suggests that Abeta-induced neurotoxicity is a conserved function of APP proteins whereby the lack of conservation in the primary sequence indicates that secondary structural aspects determine their pathogenesis. In addition, we found that the behavioral phenotypes precede extracellular amyloid deposit formation, supporting results that intracellular Abeta plays a key role in AD.
High-resolution electron microscopy of nervous systems has enabled the reconstruction of synaptic connectomes. However, we do not know the synaptic sign for each connection (i.e., whether a connection is excitatory or inhibitory), which is implied by the released transmitter. We demonstrate that artificial neural networks can predict transmitter types for presynapses from electron micrographs: a network trained to predict six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy of 87% for individual synapses, 94% for neurons, and 91% for known cell types across a D. melanogaster whole brain. We visualize the ultrastructural features used for prediction, discovering subtle but significant differences between transmitter phenotypes. We also analyze transmitter distributions across the brain and find that neurons that develop together largely express only one fast-acting transmitter (acetylcholine, glutamate, or GABA). We hope that our publicly available predictions act as an accelerant for neuroscientific hypothesis generation for the fly.
The vast majority of the adult fly ventral nerve cord is composed of 34 hemilineages, which are clusters of lineally related neurons. Neurons in these hemilineages use one of the three fast-acting neurotransmitters (acetylcholine, GABA, or glutamate) for communication. We generated a comprehensive neurotransmitter usage map for the entire ventral nerve cord. We did not find any cases of neurons using more than one neurotransmitter, but found that the acetylcholine specific gene ChAT is transcribed in many glutamatergic and GABAergic neurons, but these transcripts typically do not leave the nucleus and are not translated. Importantly, our work uncovered a simple rule: All neurons within a hemilineage use the same neurotransmitter. Thus, neurotransmitter identity is acquired at the stem cell level. Our detailed transmitter- usage/lineage identity map will be a great resource for studying the developmental basis of behavior and deciphering how neuronal circuits function to regulate behavior.