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Tools, Reagents, Data

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Stern Lab / Tools, Reagents, Data
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This page provides links to protocols, tools, reagents, and data that we have generated.
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Tools, Reagents, Data
node:field_slogan | entity_field
This page provides links to protocols, tools, reagents, and data that we have generated.
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MSG (Multiplexed Shotgun Genotyping)

Our Tn5-based protocol for library preparation:

Tn5_protein_prep_protocol

Link to GitHub repository of the latest MSG code. (Read the User's Manual!)

 

TagMap

TagMap protocol using homebrew Tn5

TagMap_protocol

 

Data

New non-melanogaster transgenic strains & attP-attB integration efficiencies

Updated July 5, 2017

Excel_database_of_strains_and_integration_efficiencies

Geneious files containing insertion sites of new transgenic strains

D. simulans and D. mauritiana insertions (38.1 Mb, updated Nov 8, 2016)

D. yakuba and D. santomea insertions (37.7 Mb, updated Nov 8, 2016)

D. virilis insertions (46.2 Mb, updated Nov 8, 2016)

 

Courtship song data from Stern 2014

Raw data from Stern, 2014. Reported Drosophila courtship song rhythms are artifacts of data analysis. BMC Biology
 
Click on the link below and a 20Gb tar file will download.
MD5 (rawData.tar) = b5d62a8082f1625494562a49acdc4970 
Segmented inter-pulse interval data. (2.5Mb file)
MD5 (IPIs.zip) = 3b0a4723083fc7fc21b4c55ec03fa5a6

​Courtship song data from Stern et al. 2017

Raw data from Stern et al. 2017. Experimental and statistical re-evaluation provides no evidence for Drosophila courtship song rhythms. PNAS
Original song recordings from Oregon-R and perL flies
Click on link below to download 3.3Gb tar file.
MD5 (Stern2017recordings.tar.gz) = 41615770b6190d47fe88c80f51630fad
Segmented songs
Click on link below to download 3.0Gb file.
MD5 (Stern2017segmentations.tar) = 7d14650a3141b50eea4fe7f8d730cce5