Main Menu (Mobile)- Block

Main Menu - Block

2 Photon Random Access Mesoscope

janelia7_blocks-janelia7_node_tool_type_list | block
Imaging Instrumentation
node_title | node_title

2 Photon Random Access Mesoscope

node:body | entity_field

About the innovation

Researchers at HHMI Janelia Research Campus have designed and built a 2-photon random access mesoscope (2p-RAM) that provides rapid access for imaging anywhere within a large tissue volume.

Cellular imaging can link activity in populations of neurons with behavior. But individual neurons are small, around 10μm diameter. Most microscopes with subcellular resolution have small fields-of-view and therefore cannot image neurons in multiple brain areas at the same time. Most large field-of-view microscopes do not resolve single cells, especially in the axial dimension.

The patent-pending 2p-RAM addresses these problems with technological advancements in remote focusing, correction of field curvature, and fast lateral and axial scanning. The complete 2p-RAM allows high-resolution imaging anywhere within a volume spanning multiple brain areas (∅ 5 mm x 1 mm cylinder) and resolution is near diffraction limited (lateral, 0.66 μm, axial 4.09 μm at the center; excitation wavelength = 970 nm; numerical aperture = 0.6) over a large range of excitation wavelengths. A fast three-dimensional scanning system allows efficient sampling of neural activity in arbitrary regions of interest across the entire imaging volume.

The 2p-RAM was made public and training offered to attendees at a Janelia 2p-RAM workshop in August 2016, where researchers learned to make and use the first 13 instruments outside of Janelia. The instrument is now available for purchase at Thorlabs, Inc. (see link to right), and the plans are available via Research License for individual academic use (see License Request to right). 

Specifications:

  • Field of view (FOV) diameter: 5.0 mm

  • Imaging volume depth: 1mm

  • Excitation numerical aperture (NA): 0.6

  • Collection numerical aperture (NA): 1.0

  • Excitation wavelengths: 900 nm-1070 nm

Patent Status:

Published U.S. Patent US-20170123196

Opportunity:

  • Commercially available from Thorlabs, Inc.
  • Documentation may be licensed for non-profit research via the Request License link at top right.
  • To learn more about the instrument, please view our documentation guidelines
  • Additional Free Mouse Headplate available here

For inquiries, please reference:

Janelia 2016-006

node:field_image | entity_field
node:field_label | entity_field
2p-RAM brain area
janelia7_blocks-janelia7_request_this_tool | block
individual_tool_resources | views
janelia7_blocks-janelia7_publication_list | block
janelia7_blocks-janelia7_tool_collaborators | block
janelia7_blocks-janelia7_program_administration | block

Contact

Michael Perham
Head of Innovation Management
addthis-addthis_block | block