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SpikeGLX Neurophysiology Probe Acquisition Software

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SpikeGLX Neurophysiology Probe Acquisition Software

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SpikeGLX is a recording system for extracellular neural probes. The emphasis is on concurrent synchronous recording from high channel count probes together with numerous auxiliary analog and digital channels:

  • Concurrent, synchronized acquisition from Imec and NI-DAQ devices.
  • Imec Neuropixels phase3A, phase3B, phase20 probe support.
  • HHMI/Whisper System support.
  • Flexible visualization, filtering, and sorting tools.
  • Programmable triggering.
  • Remote control via MATLAB.
  • Powerful offline viewing and editing.

Imec Project Phases

There are four branches in this repo separately supporting the four Imec Neuropixels project development phases. The four phases have mutually incompatible hardware and software, so obtain the appropriate materials for your needs. Release software packages are labeled as phase3A, phase3B1, phase3B2, or phase20. All early releases having no phase label are actually phase3A. All releases support NI-DAQ based acquisition.

  • Phase3A: Initially, 4 flavors of prototype probe (option 1, 2, 3, 4) were created, enabling consortium members to choose the most useful architecture and feature set. Phase3A probes are connected one at a time to a Xilinx Kintex 7 FPGA board and accessed via Ethernet. The probes, cables, HS, and BSC parts are specific to phase3A.

  • Phase3B1: At this phase, option 3 probes had won and were further developed for commercial production. The same Xilinx board is retained for one-at-a-time probe operation over Ethernet (but reprogrammed for 3B1). The cables, HS, and BSC parts are all specific to 3B1. The 3B probes can be run with either 3B1 or 3B2 setups.

  • Phase3B2: This phase replaces the Xilinx board with PXIe based modules, each of which connects up to 4 probes. Several modules can be operated together from one PXI chassis and one application. The probes are the same as the phase3B1, but all other hardware parts are specific to the PXIe implementation.

  • Phase20: Neuropixels 2.0 introduces specific probes and HS with a more compact design. The HS each supports two probes. The same PXIe modules can be used with a firmware update. Initially, SpikeGLX for this phase is specific to 2.0 hardware. In the future, one app will run 2.0 and 3B2 hardware.


SpikeGLX is developed by Bill Karsh of the Tim Harris Lab at HHMI/Janelia Research Campus.

Compiled Software

Download official release software and support materials here:

System Requirements

Requirements differ according to platform:

Frequently Asked Questions



Use is subject to Janelia Research Campus Software Copyright 1.2 license terms:

QLed components are subject to GNU Library GPL v2.0 terms, which are described here: QLed-LGPLv2-LICENSE.txt.

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