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The FuncEWorm project team is generating life-spanning FIBSEM datasets in native tissue context and integrating this ultrastructural information with single-cell RNA and ATAC sequencing. This effort will produce an unprecedented multimodal resource for the research community and establish a new paradigm for understanding complex biological systems. FuncEWorm builds on several lab collaborations and Team Projects at Janelia:
- The Shroff lab pioneered a light sheet microscopy technique that allows for individual cell registration and untwisting of live, developing C. elegans.
- The Wang lab, Flow Cytometry, and Quantitative Genomics developed transcriptomics protocols and analysis pipelines for collection of spatial ATAC and RNA sequencing data at single cell resolution.
- FIBSEM datasets are collected leveraging expertise from the EM Shared Resource, FIBSEM technology, and Shroff lab.
- The CellMap project team, Funke lab, and Scientific Computing Software have provided a framework for segmenting and analyzing dense FIBSEM data for biological insight. This relies on specialist annotation from CellMap, Project Technical Resources, and Project Pipeline Support.