In toto imaging and reconstruction of post-implantation mouse development at the single-cell level.

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Cell. 2018 Oct 10;175(3):859-876. doi: 10.1016/j.cell.2018.09.031

In toto imaging and reconstruction of post-implantation mouse development at the single-cell level. Turaga LabBranson LabKeller Lab

McDole K, Guignard L, Amat F, Berger A, Malandain G, Royer LA, Turaga SC, Branson K, Keller PJ

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Abstract

The mouse embryo has long been central to the study of mammalian development; however, elucidating the cell behaviors governing gastrulation and the formation of tissues and organs remains a fundamental challenge. A major obstacle is the lack of live imaging and image analysis technologies capable of systematically following cellular dynamics across the developing embryo. We developed a light-sheet microscope that adapts itself to the dramatic changes in size, shape, and optical properties of the post-implantation mouse embryo and captures its development from gastrulation to early organogenesis at the cellular level. We furthermore developed a computational framework for reconstructing long-term cell tracks, cell divisions, dynamic fate maps, and maps of tissue morphogenesis across the entire embryo. By jointly analyzing cellular dynamics in multiple embryos registered in space and time, we built a dynamic atlas of post-implantation mouse development that, together with our microscopy and computational methods, is provided as a resource.

PMID: 30318151 [PubMed - indexed for MEDLINE]

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In toto imaging and reconstruction of post-implantation mouse development at the single-cell level.

Abstract

PMID: 30318151 [PubMed - indexed for MEDLINE]

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