Main Menu (Mobile)- Block

Main Menu - Block

Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging.

Our Research / Publications
janelia7_blocks-janelia7_biblio_header | block
Nature Communications. 2023 Oct 27;14(1):6598. doi: 10.1038/s41467-023-42230-5
View Article on
Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging. Podgorski Lab
Nasu Y, Aggarwal A, Le GN, Vo CT, Kambe Y, Wang X, Beinlich FR, Lee AB, Ram TR, Wang F, Gorzo KA, Kamijo Y, Boisvert M, Nishinami S, Kawamura G, Ozawa T, Toda H, Gordon GR, Ge S, Hirase H, Nedergaard M, Paquet M, Drobizhev M, Podgorski K, Campbell RE
janelia7_blocks-janelia7_biblio_abstract | block

Abstract

L-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. However, investigations of the inter- and intra-cellular dynamics of L-lactate are currently hampered by the limited selection and performance of L-lactate-specific genetically encoded biosensors. Here we now report a spectrally and functionally orthogonal pair of high-performance genetically encoded biosensors: a green fluorescent extracellular L-lactate biosensor, designated eLACCO2.1, and a red fluorescent intracellular L-lactate biosensor, designated R-iLACCO1. eLACCO2.1 exhibits excellent membrane localization and robust fluorescence response. To the best of our knowledge, R-iLACCO1 and its affinity variants exhibit larger fluorescence responses than any previously reported intracellular L-lactate biosensor. We demonstrate spectrally and spatially multiplexed imaging of L-lactate dynamics by coexpression of eLACCO2.1 and R-iLACCO1 in cultured cells, and in vivo imaging of extracellular and intracellular L-lactate dynamics in mice.

PMID: 37891202 [PubMed - indexed for MEDLINE]

janelia7_blocks-janelia7_biblio_authors | block

Janelia Authors

Abhi Aggarwal
Kaspar Podgorski
janelia7_blocks-janelia7_biblio_tools | block