Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking.

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bioRxiv. 2022 May 29;. doi: 10.1101/2022.05.29.493906

Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking. Liu (Zhe) LabO'Shea LabIntegrative ImagingViral Tools

Wong V, Houlihan PR, Liu H, Walpita D, DeSantis MC, Liu Z, O’Shea E

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Abstract

AMPA-type receptors (AMPARs) are rapidly inserted into synapses undergoing long-term potentiation (LTP) to increase synaptic transmission, but how AMPAR-containing vesicles are selectively trafficked to these synapses during LTP is not known. Here we developed a strategy to label AMPAR GluA1 subunits expressed from the endogenous loci of rat hippocampal neurons such that the motion of GluA1-containing vesicles in time-lapse sequences can be characterized using single-particle tracking and mathematical modeling. We find that GluA1-containing vesicles are confined and concentrated near sites of stimulation-induced plasticity. We show that confinement is mediated by actin polymerization, which hinders the active transport of GluA1-containing vesicles along the length of the dendritic shaft by modulating the rheological properties of the cytoplasm. Actin polymerization also facilitates myosin-mediated transport of GluA1-containing vesicles to exocytic sites. We conclude that neurons utilize F-actin to increase vesicular GluA1 reservoirs and promote exocytosis proximal to the sites of neuronal activity.

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bioRxiv PrePrint https://doi.org/10.1101/2022.05.29.493906

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Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking.

Abstract

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