Rapid and semi-automated extraction of neuronal cell bodies and nuclei from electron microscopy image stacks.

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Methods in molecular biology (Clifton, N.J.). 2016;1427:277-90. doi: 10.1007/978-1-4939-3615-1_16
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Rapid and semi-automated extraction of neuronal cell bodies and nuclei from electron microscopy image stacks. FlyEMScientific Computing
Holcomb PS, Morehead M, Doretto G, Chen P, Berg S, Plaza S, Spirou G
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Abstract

Connectomics-the study of how neurons wire together in the brain-is at the forefront of modern neuroscience research. However, many connectomics studies are limited by the time and precision needed to correctly segment large volumes of electron microscopy (EM) image data. We present here a semi-automated segmentation pipeline using freely available software that can significantly decrease segmentation time for extracting both nuclei and cell bodies from EM image volumes.

PMID: 27259933 [PubMed - indexed for MEDLINE]

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Janelia Authors

Stuart Berg
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