The subiculum is a patchwork of discrete subregions.

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eLife. 2018 Oct 30;7. doi: 10.7554/eLife.37701

The subiculum is a patchwork of discrete subregions. Spruston LabQuantitative GenomicsScientific Computing SoftwareAnatomy and Histology

Cembrowski MS, Wang L, Lemire AL, Copeland M, DiLisio SF, Clements J, Spruston N

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Abstract

In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here, we investigated the extent and organizational principles governing pyramidal cell heterogeneity throughout the mouse subiculum. Using single-cell RNA-seq, we find that the subiculum pyramidal cell population can be deconstructed into eight separable subclasses. These subclasses were mapped onto abutting spatial domains, ultimately producing a complex laminar and columnar organization with heterogeneity across classical dorsal-ventral, proximal-distal, and superficial-deep axes. We further show that these transcriptomically defined subclasses correspond to differential protein products and can be associated with specific projection targets. This work deconstructs the complex landscape of subiculum pyramidal cells into spatially segregated subclasses that may be observed, controlled, and interpreted in future experiments.

PMID: 30375971 [PubMed - indexed for MEDLINE]

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The subiculum is a patchwork of discrete subregions.

Abstract

PMID: 30375971 [PubMed - indexed for MEDLINE]

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