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34 Janelia Publications

Showing 31-34 of 34 results
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    Saalfeld LabSinger Lab
    05/28/15 | BigDataViewer: visualization and processing for large image data sets.
    Pietzsch T, Saalfeld S, Preibisch S, Tomancak P
    Nature Methods. 2015 May 28;12(6):481-3. doi: 10.1038/nmeth.3392
    11/25/14 | Post-acquisition image based compensation for thickness variation in microscopy section series.
    Hanslovsky P, Bogovic JA, Saalfeld S
    IEEE International Symposium on Biomedical Imaging. 2014 Nov 25:507-11

    Serial section Microscopy is an established method for volumetric anatomy reconstruction. Section series imaged with Electron Microscopy are currently vital for the reconstruction of the synaptic connectivity of entire animal brains such as that of Drosophila melanogaster. The process of removing ultrathin layers from a solid block containing the specimen, however, is a fragile procedure and has limited precision with respect to section thickness. We have developed a method to estimate the relative z-position of each individual section as a function of signal change across the section series. First experiments show promising results on both serial section Transmission Electron Microscopy (ssTEM) data and Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) series. We made our solution available as Open Source plugins for the TrakEM2 software and the ImageJ distribution Fiji.

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    Cardona LabSaalfeld LabFetter Lab
    07/01/12 | Elastic volume reconstruction from series of ultra-thin microscopy sections.
    Saalfeld S, Fetter RD, Cardona A, Tomancak P
    Nature Methods. 2012 Jul;9(7):717-20. doi: 10.1038/nmeth.2072

    Anatomy of large biological specimens is often reconstructed from serially sectioned volumes imaged by high-resolution microscopy. We developed a method to reassemble a continuous volume from such large section series that explicitly minimizes artificial deformation by applying a global elastic constraint. We demonstrate our method on a series of transmission electron microscopy sections covering the entire 558-cell Caenorhabditis elegans embryo and a segment of the Drosophila melanogaster larval ventral nerve cord.

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    Cardona LabSaalfeld Lab
    06/02/10 | Identifying neuronal lineages of Drosophila by sequence analysis of axon tracts.
    Cardona A, Saalfeld S, Arganda I, Pereanu W, Schindelin J, Hartenstein V
    The Journal of Neuroscience. 2010 Jun 2;30(22):7538-53. doi: 10.1523/JNEUROSCI.0186-10.2010

    The Drosophila brain is formed by an invariant set of lineages, each of which is derived from a unique neural stem cell (neuroblast) and forms a genetic and structural unit of the brain. The task of reconstructing brain circuitry at the level of individual neurons can be made significantly easier by assigning neurons to their respective lineages. In this article we address the automation of neuron and lineage identification. We focused on the Drosophila brain lineages at the larval stage when they form easily recognizable secondary axon tracts (SATs) that were previously partially characterized. We now generated an annotated digital database containing all lineage tracts reconstructed from five registered wild-type brains, at higher resolution and including some that were previously not characterized. We developed a method for SAT structural comparisons based on a dynamic programming approach akin to nucleotide sequence alignment and a machine learning classifier trained on the annotated database of reference SATs. We quantified the stereotypy of SATs by measuring the residual variability of aligned wild-type SATs. Next, we used our method for the identification of SATs within wild-type larval brains, and found it highly accurate (93-99%). The method proved highly robust for the identification of lineages in mutant brains and in brains that differed in developmental time or labeling. We describe for the first time an algorithm that quantifies neuronal projection stereotypy in the Drosophila brain and use the algorithm for automatic neuron and lineage recognition.

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